A Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticles

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

5 Scopus Citations
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Author(s)

  • Yu Gao
  • Jing Lim
  • David Chen Loong Yeo
  • Shanshan Liao
  • Malin Lans
  • Yaqi Wang
  • Swee-Hin Teoh
  • Bee Tin Goh

Detail(s)

Original languageEnglish
Pages (from-to)6336-6343
Journal / PublicationACS Applied Materials and Interfaces
Volume8
Issue number10
Publication statusPublished - 16 Mar 2016
Externally publishedYes

Abstract

Cellular labeling with inorganic nanoparticles such as magnetic iron oxide nanoparticles, quantum dots, and fluorescent silica nanoparticles is an important method for the noninvasive visualization of cells using various imaging modalities. Currently, this is mainly achieved through the incubation of cultured cells with the nanoparticles that eventually reach the intracellular compartment through specific or nonspecific internalization. This classic method is advantageous in terms of simplicity and convenience, but it suffers from issues such as difficulties in fully removing free nanoparticles (suspended in solution) and the lack of selectivity on cell types. This article reports an innovative strategy for the specific labeling of adherent cells without the concern of freely suspended nanoparticles. This method relies on a nanocomposite film that is prepared by homogeneously dispersing nanoparticles within a biodegradable polymeric film. When adherent cells are seeded on the film, they adhere, spread, and filtrate into the film through the micropores formed during the film fabrication. The pre-embedded nanoparticles are thus internalized by the cells during this infiltration process. As an example, fluorescent silica nanoparticles were homogeneously distributed within a polycaprolactone film by utilizing cryomilling and heat pressing. Upon incubation within physiological buffer, no silica nanoparticles were released from the nanocomposite film even after 20 d of incubation. However, when adherent cells (e.g., human mesenchymal stem cells) were grown on the film, they became fluorescent after 3 d, which suggests internalization of silica nanoparticles by cells. In comparison, the suspension cells (e.g., monocytes) in the medium remained nonfluorescent no matter whether there was the presence of adherent cells or not. This strategy eventually allowed the selective and concomitant labeling of mesenchymal stem cells during their harvest from bone marrow aspiration.

Research Area(s)

  • selective cell labeling, nanoparticles, nanocomposite polymeric film, adherent cells, mesenchymal stem cells, bone marrow aspiration

Citation Format(s)

A Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticles. / Gao, Yu; Lim, Jing; Yeo, David Chen Loong et al.
In: ACS Applied Materials and Interfaces, Vol. 8, No. 10, 16.03.2016, p. 6336-6343.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review