A rotating microfluidic array chip for staining assays

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)1203-1208
Journal / PublicationTalanta
Volume81
Issue number4-5
Publication statusPublished - 15 Jun 2010
Externally publishedYes

Abstract

We have developed a microfluidic method to construct an array on a circular disk for staining assays. In this method, convenient centrifugal liquid pumping has been achieved within the spiral microchannels by disk rotation or spinning. Moreover, the liquids flowing in spiral channels effectively interact with the along-channel intercepted cell trapping holes. Live cells were encapsulated in wet low-melting point agarose along radial strips on the disk. When embedded in agarose, the cells remained viable to interact with, and respond to, test reagents. This method illustrates the potential use of the circular microfluidic chip to construct the cell array, intended for multi-cell multi-reagent tests. © 2010 Elsevier B.V. All rights reserved.

Research Area(s)

  • Cell array, Cell encapsulation, Centrifugal pumping, Microfluidic chip, Spiral microchannel, Staining assay

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to lbscholars@cityu.edu.hk.

Citation Format(s)

A rotating microfluidic array chip for staining assays. / Chen, Hong; Li, XiuJun; Wang, Lin; Li, Paul C.H.

In: Talanta, Vol. 81, No. 4-5, 15.06.2010, p. 1203-1208.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review