A rhodamine derivative-based fluorescent probe for visual monitoring of pH changes in the Golgi apparatus

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

1 Scopus Citations
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Author(s)

  • Zhongjiao Feng
  • Jiasheng Wu
  • Meiyu Jiang
  • Jie Sha
  • Weimin Liu
  • Haohui Ren
  • Pengfei Wang

Detail(s)

Original languageEnglish
Article number131963
Journal / PublicationSensors and Actuators B: Chemical
Volume366
Online published27 Apr 2022
Publication statusPublished - 1 Sep 2022

Abstract

The pH homeostasis of Golgi plays a vital role in maintaining normal cell functions. An effective near-infrared fluorescent probe that can sense pH changes in Golgi apparatus is of great significance. Here, a Golgi-targeting fluorescent probe (Golgi-hNR) based on a rhodamine derivative is presented to show a highly sensitive response to pH variations. Golgi-hNR displays a conspicuous pH-dependent behavior at 664 nm from pH 7.4–3.0 with a remarkable fluorescence off-on response. The pKa value of Golgi-hNR is measured to be 3.62. Confocal fluorescence imaging reveals that Golgi-hNR diffuses into Golgi with high selectivity. Using Golgi-hNR, we have successfully visualized real-time tracking of Golgi pH variations for medical therapy in living cells.

Research Area(s)

  • Fluorescent probe, Golgi apparatus, pH probe, Rhodamine derivative

Citation Format(s)

A rhodamine derivative-based fluorescent probe for visual monitoring of pH changes in the Golgi apparatus. / Feng, Zhongjiao; Wu, Jiasheng; Jiang, Meiyu; Sha, Jie; Liu, Weimin; Ren, Haohui; Zhang, Wenjun; Lee, Chun-Sing; Wang, Pengfei.

In: Sensors and Actuators B: Chemical, Vol. 366, 131963, 01.09.2022.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review