A Review of Automated Microinjection of Zebrafish Embryos

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

23 Scopus Citations
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Author(s)

  • Yuliang Zhao
  • Hui Sun
  • Xiaopeng Sha
  • Lijia Gu
  • Zhikun Zhan

Related Research Unit(s)

Detail(s)

Original languageEnglish
Article number7
Journal / PublicationMicromachines
Volume10
Issue number1
Online published24 Dec 2018
Publication statusPublished - Jan 2019

Link(s)

Abstract

Cell microinjection is a technique of precise delivery of substances into cells and is widely used for studying cell transfection, signaling pathways, and organelle functions. Microinjection of the embryos of zebrafish, the third most important animal model, has become a very useful technique in bioscience. However, factors such as the small cell size, high cell deformation tendency, and transparent zebrafish embryo membrane make the microinjection process difficult. Furthermore, this process has strict, specific requirements, such as chorion softening, avoiding contacting the first polar body, and high-precision detection. Therefore, highly accurate control and detection platforms are critical for achieving the automated microinjection of zebrafish embryos. This article reviews the latest technologies and methods used in the automated microinjection of zebrafish embryos and provides a detailed description of the current developments and applications of robotic microinjection systems. The review covers key areas related to automated embryo injection, including cell searching and location, cell position and posture adjustment, microscopic visual servoing control, sensors, actuators, puncturing mechanisms, and microinjection.

Research Area(s)

  • Automated microinjection, Cell microinjection, Microscopic visual servoing, Zebrafish embryo

Citation Format(s)

A Review of Automated Microinjection of Zebrafish Embryos. / Zhao, Yuliang; Sun, Hui; Sha, Xiaopeng; Gu, Lijia; Zhan, Zhikun; Li, Wen J.

In: Micromachines, Vol. 10, No. 1, 7, 01.2019.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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