A reversible microarray immobilization strategy based on thiol-quinone reaction

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Original languageEnglish
Journal / PublicationChinese Chemical Letters
Online published29 Jun 2021
Publication statusOnline published - 29 Jun 2021


Microarray technology has been widely applied in biomedical research. The key to microarray study is to develop efficient immobilization method. In this study, we designed a new reversible microarray immobilization method based on thiol-quinone reaction. A quinone-functionalized slide was fabricated through H2O2 treatment of dopamine-coated slides. Various thiol-containing molecules can be anchored onto the quinone-functionalized slides via thioether linker, which could be cleaved under H2O2 treatment to regenerate quinone groups on the surface. The highly versatile approach can be widely used for immobilization of various thiol-containing molecules.

Research Area(s)

  • Biochip, Peptides, Reversible Microarray, Thiol-proteins, Thiol-quinone reaction