A reverse vaccinology approach to swine dysentery vaccine development

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)111-119
Journal / PublicationVeterinary Microbiology
Volume137
Issue number1-2
Online published25 Dec 2008
Publication statusPublished - 28 May 2009
Externally publishedYes

Abstract

Swine dysentery (SD) is a mucohaemorrhagic colitis of pigs resulting from infection of the large intestine with the anaerobic intestinal spirochaete Brachyspira hyodysenteriae. Whole-cell bacterin vaccines are available to help control SD, but their performance has been inconsistent. This study aimed to use a reverse vaccinology approach to identify B. hyodysenteriae proteins for use as recombinant vaccine components. Nineteen open reading frames (ORFs) predicted to encode potential vaccine candidate molecules were identified from in silico analysis of partial genomic sequence data. The distribution of these ORFs among strains of B. hyodysenteriae was investigated by PCR, and widely distributed ORFs were cloned. The products were screened with a panel of immune pig sera, and from these a subset of conserved, immunogenic proteins was selected. Mice immunized intramuscularly with these recombinant proteins developed specific systemic antibody responses to them, and their sera agglutinated B. hyodysenteriae cells in vitro. In a pilot experiment, eight pigs were vaccinated twice intramuscularly with a combination of four of the proteins. The pigs developed antibodies to the proteins, and following experimental challenge only one developed SD compared to five of nine non-vaccinated control pigs. Although these differences in incidence were not significant, they indicated a trend towards protection using the recombinant proteins as immunogens. This study demonstrates that the reverse vaccinology approach has considerable potential for use in developing novel recombinant vaccines for SD.

Research Area(s)

  • Brachyspira hyodysenteriae, Recombinant protein, Reverse vaccinology, Spirochaete, Swine dysentery

Citation Format(s)

A reverse vaccinology approach to swine dysentery vaccine development. / Song, Yong; La, Tom; Phillips, Nyree D. et al.
In: Veterinary Microbiology, Vol. 137, No. 1-2, 28.05.2009, p. 111-119.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review