A reduced graphene oxide-Au based electrochemical biosensor for ultrasensitive detection of enzymatic activity of botulinum neurotoxin A

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

24 Scopus Citations
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Author(s)

  • Chun-Yu Chan
  • Jiubiao Guo
  • Cheng Sun
  • Ming-Kiu Tsang
  • Feng Tian
  • Jianhua Hao
  • Mo Yang

Detail(s)

Original languageEnglish
Pages (from-to)131-137
Journal / PublicationSensors and Actuators, B: Chemical
Volume220
Online published31 May 2015
Publication statusPublished - 1 Dec 2015
Externally publishedYes

Abstract

Botulinum neurotoxin serotype A (BoNT/A) is a type of neurotoxin which is able to cause fatal paralytic illness botulism in a low dosage. Therefore, there is a great need to develop an ultrasensitive bioassay to detect its active state for early diagnostics and prevention. This paper presents a reduced graphene oxide (rGO)/Au electrode based electrochemical biosensor for ultrasensitive detection of BoNT serotype A light chain (BoNT-LcA) protease activity. The fabricated rGO/Au electrode provides a robust and biocompatible platform with enhanced electron transfer capability and large area for peptide immobilization. SNAP-25-GFP peptide substrate is firstly immobilized on rGO surface via pyrenebutyric acid (PA) linker. The addition of BoNT-LcA could specifically cut SNAP-25-GFP at the cleavage sites to release the cut section from the electrode surface. This enzymatic activity of BoNT-LcA on SNAP-25-GFP peptide substrate could be detected by monitoring the enhanced redox probe transfer rate by differential pulse voltammetry (DPV) with a linear detection range from 1 pg/mL to 1 ng/mL and the limit of detection (LOD) for BoNT-LcA is around 8.6 pg/mL. The specificity of this biosensor is demonstrated with BoNT-LcB and heat-treated BoNT-LcA. Moreover, the experiments for BoNT-LcA detection in milk samples demonstrate the feasibility of this biosensor in complex matrix.

Research Area(s)

  • Botulinum neurotoxin type A (BoNT/A), Differential pulse voltammetry (DPV), Electrochemical biosensor, Reduced graphene oxide (rGO)

Citation Format(s)

A reduced graphene oxide-Au based electrochemical biosensor for ultrasensitive detection of enzymatic activity of botulinum neurotoxin A. / Chan, Chun-Yu; Guo, Jiubiao; Sun, Cheng; Tsang, Ming-Kiu; Tian, Feng; Hao, Jianhua; Chen, Sheng; Yang, Mo.

In: Sensors and Actuators, B: Chemical, Vol. 220, 01.12.2015, p. 131-137.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review