A ratiometric fluorescent probe for single and two-photon bioimaging of hydrogen peroxide in vitro and in vivo

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Zhongjiao Feng
  • Jiasheng Wu
  • Weimin Liu
  • Haohui Ren
  • Xiuli Zheng
  • Pengfei Wang

Detail(s)

Original languageEnglish
Article number133668
Journal / PublicationSensors and Actuators B: Chemical
Volume384
Online published13 Mar 2023
Publication statusPublished - 1 Jun 2023

Abstract

Hydrogen peroxide (H2O2) as an important reactive oxygen species (ROS) is correlated in various oxidative stress and cell signaling processes. Thus, developing an effective method that can monitor H2O2 in the Golgi apparatus is of great importance. Herein, a Golgi-targeting two-photon fluorescent probe (GT-H2O2) that can be excited by a femto-second pulse (760 nm) is designed. GT-H2O2 utilized aminoquinoline as a fluorophore / Golgi-targetable unit and borate ester as a H2O2-responsive unit with an intramolecular charge transfer strategy. The borate ester of GT-H2O2 was oxidized by H2O2 selectively to afford a new fluorescent molecule GT-NH2, thereby realizing a ratiometric detection of H2O2. The probe exhibited a high sensitivity and selectivity to H2O2 with a linear range from 5 to 60 μM. In addition, endogenous / exogenous H2O2 could be directly visualized utilizing GT-H2O2 in vitro and in vivo by one/two-photon microscopy. © 2023 Elsevier B.V.

Research Area(s)

  • Aminoquinoline derivative, Golgi apparatus, Hydrogen peroxide, Ratiometric fluorescent probe, Two-photon imaging

Citation Format(s)

A ratiometric fluorescent probe for single and two-photon bioimaging of hydrogen peroxide in vitro and in vivo. / Feng, Zhongjiao; Wu, Jiasheng; Liu, Weimin et al.
In: Sensors and Actuators B: Chemical, Vol. 384, 133668, 01.06.2023.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review