TY - JOUR
T1 - A ratiometric fluorescent probe for single and two-photon bioimaging of hydrogen peroxide in vitro and in vivo
AU - Feng, Zhongjiao
AU - Wu, Jiasheng
AU - Liu, Weimin
AU - Ren, Haohui
AU - Zheng, Xiuli
AU - Zhang, Wenjun
AU - Lee, Chun-Sing
AU - Wang, Pengfei
PY - 2023/6/1
Y1 - 2023/6/1
N2 - Hydrogen peroxide (H2O2) as an important reactive oxygen species (ROS) is correlated in various oxidative stress and cell signaling processes. Thus, developing an effective method that can monitor H2O2 in the Golgi apparatus is of great importance. Herein, a Golgi-targeting two-photon fluorescent probe (GT-H2O2) that can be excited by a femto-second pulse (760 nm) is designed. GT-H2O2 utilized aminoquinoline as a fluorophore / Golgi-targetable unit and borate ester as a H2O2-responsive unit with an intramolecular charge transfer strategy. The borate ester of GT-H2O2 was oxidized by H2O2 selectively to afford a new fluorescent molecule GT-NH2, thereby realizing a ratiometric detection of H2O2. The probe exhibited a high sensitivity and selectivity to H2O2 with a linear range from 5 to 60 μM. In addition, endogenous / exogenous H2O2 could be directly visualized utilizing GT-H2O2 in vitro and in vivo by one/two-photon microscopy. © 2023 Elsevier B.V.
AB - Hydrogen peroxide (H2O2) as an important reactive oxygen species (ROS) is correlated in various oxidative stress and cell signaling processes. Thus, developing an effective method that can monitor H2O2 in the Golgi apparatus is of great importance. Herein, a Golgi-targeting two-photon fluorescent probe (GT-H2O2) that can be excited by a femto-second pulse (760 nm) is designed. GT-H2O2 utilized aminoquinoline as a fluorophore / Golgi-targetable unit and borate ester as a H2O2-responsive unit with an intramolecular charge transfer strategy. The borate ester of GT-H2O2 was oxidized by H2O2 selectively to afford a new fluorescent molecule GT-NH2, thereby realizing a ratiometric detection of H2O2. The probe exhibited a high sensitivity and selectivity to H2O2 with a linear range from 5 to 60 μM. In addition, endogenous / exogenous H2O2 could be directly visualized utilizing GT-H2O2 in vitro and in vivo by one/two-photon microscopy. © 2023 Elsevier B.V.
KW - Aminoquinoline derivative
KW - Golgi apparatus
KW - Hydrogen peroxide
KW - Ratiometric fluorescent probe
KW - Two-photon imaging
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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85150377164&origin=recordpage
U2 - 10.1016/j.snb.2023.133668
DO - 10.1016/j.snb.2023.133668
M3 - RGC 21 - Publication in refereed journal
SN - 0925-4005
VL - 384
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
M1 - 133668
ER -