A quantitative fluorescence intensity method to measure sub-picogram injection amount for tiny cell injection

Y. T. Chow; S. Chen; D. Sun; C. W. Kong; R. A. Li; S. H. Cheng

doi:10.1109/NANO.2015.7388793

A quantitative fluorescence intensity method to measure sub-picogram injection amount for tiny cell injection

Y. T. Chow
, S. Chen
, D. Sun^*
, C. W. Kong
, R. A. Li
, S. H. Cheng

^*Corresponding author for this work

Department of Biomedical Sciences

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

1 Citation (Scopus)

Abstract

We present a method for characterizing injection volume of the robotic cell injection system through measuring fluorescence intensity of the injected cell. The relationship of the concentration of the fluorescence dye and the fluorescence intensity was found to be linear, which was confirmed by measuring the cell-morphology-like droplets. The droplet was generated by dispensing in a mineral oil filled microfluidic cell holder. Human foreskin fibroblast (HFF) was injected with known concentration of fluorescence dye. The injection amount was determined by relating the fluorescence intensity of the injected cell and that of the droplet in mineral oil. The measured injection amount was compared with the volumetric change of the droplet to verify the accuracy of the proposed method.

Original language	English
Title of host publication	IEEE-NANO 2015 - 15th International Conference on Nanotechnology
Publisher	IEEE
Pages	1019-1022
ISBN (Print)	9781467381550
DOIs	https://doi.org/10.1109/NANO.2015.7388793
Publication status	Published - 27 Jul 2015
Event	15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015 - Angelicum Congress Centre, Rome, Italy Duration: 27 Jul 2015 → 30 Jul 2015 http://www.ieeenano15.org/

Conference

Conference	15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015
Place	Italy
City	Rome
Period	27/07/15 → 30/07/15
Internet address	http://www.ieeenano15.org/

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 9 Industry, Innovation, and Infrastructure

Research Keywords

fluorescence microscopy
microfluidics
Quantitative microinjection

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10.1109/NANO.2015.7388793

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@inproceedings{08aa6bcf794a48a2b5b7599edb007741,

title = "A quantitative fluorescence intensity method to measure sub-picogram injection amount for tiny cell injection",

abstract = "We present a method for characterizing injection volume of the robotic cell injection system through measuring fluorescence intensity of the injected cell. The relationship of the concentration of the fluorescence dye and the fluorescence intensity was found to be linear, which was confirmed by measuring the cell-morphology-like droplets. The droplet was generated by dispensing in a mineral oil filled microfluidic cell holder. Human foreskin fibroblast (HFF) was injected with known concentration of fluorescence dye. The injection amount was determined by relating the fluorescence intensity of the injected cell and that of the droplet in mineral oil. The measured injection amount was compared with the volumetric change of the droplet to verify the accuracy of the proposed method.",

keywords = "fluorescence microscopy, microfluidics, Quantitative microinjection",

author = "Chow, \{Y. T.\} and S. Chen and D. Sun and Kong, \{C. W.\} and Li, \{R. A.\} and Cheng, \{S. H.\}",

year = "2015",

month = jul,

day = "27",

doi = "10.1109/NANO.2015.7388793",

language = "English",

isbn = "9781467381550",

pages = "1019--1022",

booktitle = "IEEE-NANO 2015 - 15th International Conference on Nanotechnology",

publisher = "IEEE",

address = "United States",

note = "15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015 ; Conference date: 27-07-2015 Through 30-07-2015",

url = "http://www.ieeenano15.org/",

}

Chow, YT, Chen, S, Sun, D, Kong, CW, Li, RA & Cheng, SH 2015, A quantitative fluorescence intensity method to measure sub-picogram injection amount for tiny cell injection. in IEEE-NANO 2015 - 15th International Conference on Nanotechnology., 7388793, IEEE, pp. 1019-1022, 15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015, Rome, Italy, 27/07/15. https://doi.org/10.1109/NANO.2015.7388793

A quantitative fluorescence intensity method to measure sub-picogram injection amount for tiny cell injection. / Chow, Y. T.; Chen, S.; Sun, D. et al.
IEEE-NANO 2015 - 15th International Conference on Nanotechnology. IEEE, 2015. p. 1019-1022 7388793.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - A quantitative fluorescence intensity method to measure sub-picogram injection amount for tiny cell injection

AU - Chow, Y. T.

AU - Chen, S.

AU - Sun, D.

AU - Kong, C. W.

AU - Li, R. A.

AU - Cheng, S. H.

PY - 2015/7/27

Y1 - 2015/7/27

N2 - We present a method for characterizing injection volume of the robotic cell injection system through measuring fluorescence intensity of the injected cell. The relationship of the concentration of the fluorescence dye and the fluorescence intensity was found to be linear, which was confirmed by measuring the cell-morphology-like droplets. The droplet was generated by dispensing in a mineral oil filled microfluidic cell holder. Human foreskin fibroblast (HFF) was injected with known concentration of fluorescence dye. The injection amount was determined by relating the fluorescence intensity of the injected cell and that of the droplet in mineral oil. The measured injection amount was compared with the volumetric change of the droplet to verify the accuracy of the proposed method.

AB - We present a method for characterizing injection volume of the robotic cell injection system through measuring fluorescence intensity of the injected cell. The relationship of the concentration of the fluorescence dye and the fluorescence intensity was found to be linear, which was confirmed by measuring the cell-morphology-like droplets. The droplet was generated by dispensing in a mineral oil filled microfluidic cell holder. Human foreskin fibroblast (HFF) was injected with known concentration of fluorescence dye. The injection amount was determined by relating the fluorescence intensity of the injected cell and that of the droplet in mineral oil. The measured injection amount was compared with the volumetric change of the droplet to verify the accuracy of the proposed method.

KW - fluorescence microscopy

KW - microfluidics

KW - Quantitative microinjection

UR - https://www.scopus.com/pages/publications/84964345938

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84964345938&origin=recordpage

U2 - 10.1109/NANO.2015.7388793

DO - 10.1109/NANO.2015.7388793

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781467381550

SP - 1019

EP - 1022

BT - IEEE-NANO 2015 - 15th International Conference on Nanotechnology

PB - IEEE

T2 - 15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015

Y2 - 27 July 2015 through 30 July 2015

ER -

A quantitative fluorescence intensity method to measure sub-picogram injection amount for tiny cell injection

Abstract

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