A proposed mechanism of the influence of gold nanoparticles on DNA hybridization

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)6765-6777
Journal / PublicationACS Nano
Volume8
Issue number7
Publication statusPublished - 22 Jul 2014
Externally publishedYes

Abstract

A combination of gold nanoparticles (AuNPs) and nucleic acids has been used in biosensing applications. However, there is a poor fundamental understanding of how gold nanoparticle surfaces influence the DNA hybridization process. Here, we measured the rate constants of the hybridization and dehybridization of DNA on gold nanoparticle surfaces to enable the determination of activation parameters using transition state theory. We show that the target bases need to be detached from the gold nanoparticle surfaces before zipping. This causes a shift of the rate-limiting step of hybridization to the mismatch-sensitive zipping step. Furthermore, our results propose that the binding of gold nanoparticles to the single-stranded DNA segments (commonly known as bubbles) in the duplex DNA stabilizes the bubbles and accelerates the dehybridization process. We employ the proposed mechanism of DNA hybridization/dehybridization to explain the ability of 5 nm diameter gold nanoparticles to help discriminate between single base-pair mismatched DNA molecules when performed in a NanoBioArray chip. The mechanistic insight into the DNA-gold nanoparticle hybridization/dehybridization process should lead to the development of new biosensors. © 2014 American Chemical Society.

Research Area(s)

  • DNA hybridization and dehybridization, gold nanoparticle (AuNP), Langmuir model, NanoBioArray (NBA) chip, single base-pair discrimination, surface plasmon resonance (SPR) spectroscopy, transition state theory

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to lbscholars@cityu.edu.hk.

Citation Format(s)

A proposed mechanism of the influence of gold nanoparticles on DNA hybridization. / Sedighi, Abootaleb; Li, Paul C. H.; Pekcevik, Idah C.; Gates, Byron D.

In: ACS Nano, Vol. 8, No. 7, 22.07.2014, p. 6765-6777.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review