A Productive Expression Platform Derived from Host-Restricted Eilat Virus : Its Extensive Validation and Novel Strategy
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
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Detail(s)
Original language | English |
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Article number | 660 |
Number of pages | 17 |
Journal / Publication | Viruses |
Volume | 13 |
Issue number | 4 |
Online published | 11 Apr 2021 |
Publication status | Published - Apr 2021 |
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DOI | DOI |
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Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-85105113736&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(3684583b-9149-4678-955d-c5664fd3b431).html |
Abstract
Most alphaviruses are transmitted by mosquitoes and infect a wide range of insects and
vertebrates. However, Eilat virus (EILV) is defective for infecting vertebrate cells at multiple levels
of the viral life cycle. This host-restriction property renders EILV an attractive expression platform
since it is not infectious for vertebrates and therefore provides a highly advantageous safety profile.
Here, we investigated the feasibility of versatile EILV-based expression vectors. By replacing the
structural genes of EILV with those of other alphaviruses, we generated seven different chimeras.
These chimeras were readily rescued in the original mosquito cells and were able to reach high
titers, suggesting that EILV is capable of packaging the structural proteins of different lineages. We
also explored the ability of EILV to express authentic antigens via double subgenomic (SG) RNA
vectors. Four foreign genetic materials of varied length were introduced into the EILV genome, and
the expressed heterologous genetic materials were readily detected in the infected cells. By inserting
an additional SG promoter into the chimera genome containing the structural genes of Chikungunya
virus (CHIKV), we developed a bivalent vaccine candidate against CHIKV and Zika virus. These
data demonstrate the outstanding compatibility of the EILV genome. The produced recombinants
can be applied to vaccine and diagnostic tool development, but more investigations are required.
Research Area(s)
- Eilat virus, expression platform, insect-specific, diagnostic tool, vaccine candidate
Citation Format(s)
A Productive Expression Platform Derived from Host-Restricted Eilat Virus: Its Extensive Validation and Novel Strategy. / Tan, Lu; Zhang, Yiwen; Wang, Xingxing et al.
In: Viruses, Vol. 13, No. 4, 660, 04.2021.
In: Viruses, Vol. 13, No. 4, 660, 04.2021.
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
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