A photoelectrochemical biosensor for rapid and ultrasensitive norovirus detection

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

1 Scopus Citations
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Author(s)

  • Jiubiao Guo
  • Dan Liu
  • Zhiqiang Yang
  • Wenchuan Weng
  • Edward Wai Chi Chan
  • Zhenling Zeng
  • Kwok-Yin Wong
  • Peng Lin

Detail(s)

Original languageEnglish
Article number107591
Journal / PublicationBioelectrochemistry
Volume136
Online published1 Jul 2020
Publication statusOnline published - 1 Jul 2020

Abstract

The highly contagious norovirus (NoV) is the most common causative agent of acute gastroenteritis, resulting in >200,000 deaths worldwide annually. A rapid and sensitive detection method is a prerequisite for effective prevention and timely identification of NoV contamination. In the present study, we developed a photoelectrochemical (PEC) biosensor coupled with a novel custom-made monoclonal antibody (mAb) for specific and sensitive NoV detection. Our system could detect levels of recombinant NoV capsid protein VP1 as low as 2 × 10−10 g mL−1 (4.9 pM) within 30 min in a concentration-dependent manner. More importantly, the biosensor was versatile in detecting virus isolated from real samples that were as low as 46 copies μL−1. These findings indicate that this system has the potential to serve as a convenient point-of-care system for diagnosing NoV infection and detecting NoV-contaminated food samples.

Research Area(s)

  • Monoclonal antibody, Norovirus, Photoelectrochemical biosensor, QD, VP1

Citation Format(s)

A photoelectrochemical biosensor for rapid and ultrasensitive norovirus detection. / Guo, Jiubiao; Liu, Dan; Yang, Zhiqiang; Weng, Wenchuan; Chan, Edward Wai Chi; Zeng, Zhenling; Wong, Kwok-Yin; Lin, Peng; Chen, Sheng.

In: Bioelectrochemistry, Vol. 136, 107591, 12.2020.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal