A PH domain in ACAP1 possesses key features of the BAR domain in promoting membrane curvature

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

24 Scopus Citations
View graph of relations

Author(s)

  • Xiaoyun Pang
  • Yan Zhang
  • Kai Zhang
  • Bingquan Gao
  • Jun Ma
  • Jian Li
  • Yuchen Deng
  • Qiangjun Zhou
  • Edward H. Egelman
  • Victor W. Hsu
  • Fei Sun

Detail(s)

Original languageEnglish
Pages (from-to)73-86
Journal / PublicationDevelopmental Cell
Volume31
Issue number1
Online published2 Oct 2014
Publication statusPublished - 13 Oct 2014

Abstract

The BAR (Bin-Amphiphysin-Rvs) domain undergoes dimerization to produce a curved protein structure, which superimposes onto membrane through electrostatic interactions to sense and impart membrane curvature. In some cases, a BAR domain also possesses an amphipathic helix that inserts into the membrane to induce curvature. ACAP1 (Arfgap with Coil coil, Ankyrin repeat, and PH domain protein 1) contains a BAR domain. Here, we show that this BAR domain can neither bind membrane nor impart curvature, but instead requires a neighboring PH (Pleckstrin Homology) domain to achieve these functions. Specific residues within the PH domain are responsible for both membrane binding and curvature generation. The BAR domain adjacent to the PH domain instead interacts with the BAR domains of neighboring ACAP1 proteins to enable clustering at the membrane. Thus, we have uncovered the molecular basis for an unexpected and unconventional collaboration between PH and BAR domains in membrane bending.

Citation Format(s)

A PH domain in ACAP1 possesses key features of the BAR domain in promoting membrane curvature. / Pang, Xiaoyun; Fan, Jun; Zhang, Yan; Zhang, Kai; Gao, Bingquan; Ma, Jun; Li, Jian; Deng, Yuchen; Zhou, Qiangjun; Egelman, Edward H.; Hsu, Victor W.; Sun, Fei.

In: Developmental Cell, Vol. 31, No. 1, 13.10.2014, p. 73-86.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal