A novel magnetic tweezers for manipulation of a single DNA molecule
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Pages (from-to) | 613-616 |
Journal / Publication | Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) |
Publication status | Published - 2004 |
Externally published | Yes |
Conference
Title | 17th IEEE International Conference on Micro Electro Mechanical Systems (MEMS): Maastricht MEMS 2004 Technical Digest |
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Place | Netherlands |
City | Maastricht |
Period | 25 - 29 January 2004 |
Link(s)
Abstract
We report a novel magnetic tweezers for manipulation of a single DNA molecule. The micromachined DNA manipulator can stretch and rotate a single DNA molecule using arrayed microcoils. Key platform technologies including localized DNA immobilization, microcoil fabrication and microfluidics, have been integrated to form the magnetic DNA tweezers. A single DNA molecule is specifically attached onto a magnetic bead and a gold surface and manipulated under a magnetic field generated by built-in hexagonally-aligned microcoils. A highly effective method for the construction of DNA two sticky ends is developed, which is compatible with MEMS technologies. We have successfully demonstrated the rotation of the tethered-bead DNA molecule linked to the gold pattern by circular permutation of the currents applied to the microcoils.
Bibliographic Note
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Citation Format(s)
A novel magnetic tweezers for manipulation of a single DNA molecule. / Chiou, Chi-Han; Tseng, Zhao-Fu; Lee, Gwo-Bin.
In: Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 2004, p. 613-616.
In: Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 2004, p. 613-616.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review