A novel magnetic tweezers for manipulation of a single DNA molecule

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)613-616
Journal / PublicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Publication statusPublished - 2004
Externally publishedYes

Conference

Title17th IEEE International Conference on Micro Electro Mechanical Systems (MEMS): Maastricht MEMS 2004 Technical Digest
PlaceNetherlands
CityMaastricht
Period25 - 29 January 2004

Abstract

We report a novel magnetic tweezers for manipulation of a single DNA molecule. The micromachined DNA manipulator can stretch and rotate a single DNA molecule using arrayed microcoils. Key platform technologies including localized DNA immobilization, microcoil fabrication and microfluidics, have been integrated to form the magnetic DNA tweezers. A single DNA molecule is specifically attached onto a magnetic bead and a gold surface and manipulated under a magnetic field generated by built-in hexagonally-aligned microcoils. A highly effective method for the construction of DNA two sticky ends is developed, which is compatible with MEMS technologies. We have successfully demonstrated the rotation of the tethered-bead DNA molecule linked to the gold pattern by circular permutation of the currents applied to the microcoils.

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