A novel ESKAPE-sensitive peptide with enhanced stability and its application in controlling multiple bacterial contaminations in chilled fresh pork

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

5 Scopus Citations
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Author(s)

  • Ping Zeng
  • Pengfei Zhang
  • Lanhua Yi
  • Kwok-Yin Wong
  • Kin-Fai Chan
  • Sharon Shui Yee Leung

Detail(s)

Original languageEnglish
Article number135647
Journal / PublicationFood Chemistry
Volume413
Online published9 Feb 2023
Publication statusPublished - 1 Jul 2023

Abstract

The co-existence of various pathogenic bacteria on the surface of pork products exacerbates difficulties in food safety control. Developing broad-spectrum and stable antibacterial agents that are not antibiotics is an unmet need. To address this issue, all L-arginine residues of a reported peptide (IIRR)4-NH2 (zp80) were substituted with the corresponding D enantiomers. This novel peptide (IIrr)4-NH2 (zp80r) was expected to maintain favourable bioactivity against ESKAPE strains and have enhanced proteolytic stability compared with zp80. In a series of experiments, zp80r maintained favourable bioactivities against starvation-induced persisters. Electron microscopy and fluorescent dye assays were used to verify the antibacterial mechanism of zp80r. Importantly, zp80r reduced bacterial colonies in chilled fresh pork contaminated with multiple bacterial species. This newly designed peptide is a potential antibacterial candidate to combat problematic foodborne pathogens during storage of pork. © 2023 Elsevier Ltd

Research Area(s)

  • Broad-spectrum antibacterial, Foodborne pathogen, Helical peptide, Membrane targeting, Proteolytic stability

Citation Format(s)

A novel ESKAPE-sensitive peptide with enhanced stability and its application in controlling multiple bacterial contaminations in chilled fresh pork. / Zeng, Ping; Zhang, Pengfei; Yi, Lanhua et al.
In: Food Chemistry, Vol. 413, 135647, 01.07.2023.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review