A novel ESKAPE-sensitive peptide with enhanced stability and its application in controlling multiple bacterial contaminations in chilled fresh pork
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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Related Research Unit(s)
Detail(s)
Original language | English |
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Article number | 135647 |
Journal / Publication | Food Chemistry |
Volume | 413 |
Online published | 9 Feb 2023 |
Publication status | Published - 1 Jul 2023 |
Link(s)
Abstract
The co-existence of various pathogenic bacteria on the surface of pork products exacerbates difficulties in food safety control. Developing broad-spectrum and stable antibacterial agents that are not antibiotics is an unmet need. To address this issue, all L-arginine residues of a reported peptide (IIRR)4-NH2 (zp80) were substituted with the corresponding D enantiomers. This novel peptide (IIrr)4-NH2 (zp80r) was expected to maintain favourable bioactivity against ESKAPE strains and have enhanced proteolytic stability compared with zp80. In a series of experiments, zp80r maintained favourable bioactivities against starvation-induced persisters. Electron microscopy and fluorescent dye assays were used to verify the antibacterial mechanism of zp80r. Importantly, zp80r reduced bacterial colonies in chilled fresh pork contaminated with multiple bacterial species. This newly designed peptide is a potential antibacterial candidate to combat problematic foodborne pathogens during storage of pork. © 2023 Elsevier Ltd
Research Area(s)
- Broad-spectrum antibacterial, Foodborne pathogen, Helical peptide, Membrane targeting, Proteolytic stability
Citation Format(s)
A novel ESKAPE-sensitive peptide with enhanced stability and its application in controlling multiple bacterial contaminations in chilled fresh pork. / Zeng, Ping; Zhang, Pengfei; Yi, Lanhua et al.
In: Food Chemistry, Vol. 413, 135647, 01.07.2023.
In: Food Chemistry, Vol. 413, 135647, 01.07.2023.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review