A novel ESKAPE-sensitive peptide with enhanced stability and its application in controlling multiple bacterial contaminations in chilled fresh pork

Ping Zeng; Pengfei Zhang; Lanhua Yi; Kwok-Yin Wong; Sheng Chen; Kin-Fai Chan; Sharon Shui Yee Leung

doi:10.1016/j.foodchem.2023.135647

A novel ESKAPE-sensitive peptide with enhanced stability and its application in controlling multiple bacterial contaminations in chilled fresh pork

Ping Zeng, Pengfei Zhang, Lanhua Yi, Kwok-Yin Wong, Sheng Chen, Kin-Fai Chan^*, Sharon Shui Yee Leung^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

8 Citations (Scopus)

Abstract

The co-existence of various pathogenic bacteria on the surface of pork products exacerbates difficulties in food safety control. Developing broad-spectrum and stable antibacterial agents that are not antibiotics is an unmet need. To address this issue, all L-arginine residues of a reported peptide (IIRR)₄-NH₂ (zp80) were substituted with the corresponding D enantiomers. This novel peptide (IIrr)₄-NH₂ (zp80r) was expected to maintain favourable bioactivity against ESKAPE strains and have enhanced proteolytic stability compared with zp80. In a series of experiments, zp80r maintained favourable bioactivities against starvation-induced persisters. Electron microscopy and fluorescent dye assays were used to verify the antibacterial mechanism of zp80r. Importantly, zp80r reduced bacterial colonies in chilled fresh pork contaminated with multiple bacterial species. This newly designed peptide is a potential antibacterial candidate to combat problematic foodborne pathogens during storage of pork. © 2023 Elsevier Ltd

Original language	English
Article number	135647
Journal	Food Chemistry
Volume	413
Online published	9 Feb 2023
DOIs	https://doi.org/10.1016/j.foodchem.2023.135647
Publication status	Published - 1 Jul 2023

Funding

We are thankful to Prof. Lin Zhang and Dr. Judeng Zeng for providing the GES-1 cell line. We are thankful to Prof. Chris Lai for providing the two EF strains. We are thankful to Dr. Yu Wai Chen for analyzing the 3D structure of peptide zp80r. This work was supported by the Research Grants Council of Hong Kong (Grant 531 No. C5026-16G) and the Health and Medical Research Fund Hong Kong (Grant No. 21200782).

Research Keywords

Broad-spectrum antibacterial
Foodborne pathogen
Helical peptide
Membrane targeting
Proteolytic stability

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RGC-funded

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title = "A novel ESKAPE-sensitive peptide with enhanced stability and its application in controlling multiple bacterial contaminations in chilled fresh pork",

abstract = "The co-existence of various pathogenic bacteria on the surface of pork products exacerbates difficulties in food safety control. Developing broad-spectrum and stable antibacterial agents that are not antibiotics is an unmet need. To address this issue, all L-arginine residues of a reported peptide (IIRR)4-NH2 (zp80) were substituted with the corresponding D enantiomers. This novel peptide (IIrr)4-NH2 (zp80r) was expected to maintain favourable bioactivity against ESKAPE strains and have enhanced proteolytic stability compared with zp80. In a series of experiments, zp80r maintained favourable bioactivities against starvation-induced persisters. Electron microscopy and fluorescent dye assays were used to verify the antibacterial mechanism of zp80r. Importantly, zp80r reduced bacterial colonies in chilled fresh pork contaminated with multiple bacterial species. This newly designed peptide is a potential antibacterial candidate to combat problematic foodborne pathogens during storage of pork. {\textcopyright} 2023 Elsevier Ltd",

keywords = "Broad-spectrum antibacterial, Foodborne pathogen, Helical peptide, Membrane targeting, Proteolytic stability",

author = "Ping Zeng and Pengfei Zhang and Lanhua Yi and Kwok-Yin Wong and Sheng Chen and Kin-Fai Chan and Leung, {Sharon Shui Yee}",

year = "2023",

month = jul,

day = "1",

doi = "10.1016/j.foodchem.2023.135647",

language = "English",

volume = "413",

journal = "Food Chemistry",

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T1 - A novel ESKAPE-sensitive peptide with enhanced stability and its application in controlling multiple bacterial contaminations in chilled fresh pork

AU - Zeng, Ping

AU - Zhang, Pengfei

AU - Yi, Lanhua

AU - Wong, Kwok-Yin

AU - Chen, Sheng

AU - Chan, Kin-Fai

AU - Leung, Sharon Shui Yee

PY - 2023/7/1

Y1 - 2023/7/1

N2 - The co-existence of various pathogenic bacteria on the surface of pork products exacerbates difficulties in food safety control. Developing broad-spectrum and stable antibacterial agents that are not antibiotics is an unmet need. To address this issue, all L-arginine residues of a reported peptide (IIRR)4-NH2 (zp80) were substituted with the corresponding D enantiomers. This novel peptide (IIrr)4-NH2 (zp80r) was expected to maintain favourable bioactivity against ESKAPE strains and have enhanced proteolytic stability compared with zp80. In a series of experiments, zp80r maintained favourable bioactivities against starvation-induced persisters. Electron microscopy and fluorescent dye assays were used to verify the antibacterial mechanism of zp80r. Importantly, zp80r reduced bacterial colonies in chilled fresh pork contaminated with multiple bacterial species. This newly designed peptide is a potential antibacterial candidate to combat problematic foodborne pathogens during storage of pork. © 2023 Elsevier Ltd

AB - The co-existence of various pathogenic bacteria on the surface of pork products exacerbates difficulties in food safety control. Developing broad-spectrum and stable antibacterial agents that are not antibiotics is an unmet need. To address this issue, all L-arginine residues of a reported peptide (IIRR)4-NH2 (zp80) were substituted with the corresponding D enantiomers. This novel peptide (IIrr)4-NH2 (zp80r) was expected to maintain favourable bioactivity against ESKAPE strains and have enhanced proteolytic stability compared with zp80. In a series of experiments, zp80r maintained favourable bioactivities against starvation-induced persisters. Electron microscopy and fluorescent dye assays were used to verify the antibacterial mechanism of zp80r. Importantly, zp80r reduced bacterial colonies in chilled fresh pork contaminated with multiple bacterial species. This newly designed peptide is a potential antibacterial candidate to combat problematic foodborne pathogens during storage of pork. © 2023 Elsevier Ltd

KW - Broad-spectrum antibacterial

KW - Foodborne pathogen

KW - Helical peptide

KW - Membrane targeting

KW - Proteolytic stability

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U2 - 10.1016/j.foodchem.2023.135647

DO - 10.1016/j.foodchem.2023.135647

M3 - RGC 21 - Publication in refereed journal

C2 - 36796263

SN - 0308-8146

VL - 413

JO - Food Chemistry

JF - Food Chemistry

M1 - 135647

ER -

A novel ESKAPE-sensitive peptide with enhanced stability and its application in controlling multiple bacterial contaminations in chilled fresh pork

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CRF: Development of Novel Inhibitors Targeting the Resistance Mechanisms of Clinical Superbugs

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A novel ESKAPE-sensitive peptide with enhanced stability and its application in controlling multiple bacterial contaminations in chilled fresh pork

Abstract

Funding

Research Keywords

RGC Funding Information

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CRF: Development of Novel Inhibitors Targeting the Resistance Mechanisms of Clinical Superbugs

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