A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating

Yan-Xiang Ni; Nan Zhou; Wen-Qian Xue; Li Rong; Wing-Ho Yung; Rao-Zhou Lin; Richard Yi-Tsun Kao; Zhi-Gang Duan; Hai-Tao Sun; Hua-Rui Gong; Xu-Ming Tang; Meng-Fei Liu; Wen Zhang; Shuang Qi; Sookja Chung; You-Qiang Song; Jian-Dong Huang

doi:10.1038/s41421-018-0067-5

A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating

Yan-Xiang Ni
, Nan Zhou
, Wen-Qian Xue
, Li Rong
, Wing-Ho Yung
, Rao-Zhou Lin
, Richard Yi-Tsun Kao
, Zhi-Gang Duan
, Hai-Tao Sun
, Hua-Rui Gong
, Xu-Ming Tang
, Meng-Fei Liu
, Wen Zhang
, Shuang Qi
, Sookja Chung
, You-Qiang Song
, Jian-Dong Huang^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

5 Citations (Scopus)

42 Downloads (CityUHK Scholars)

Abstract

Kif5b-driven anterograde transport and clathrin-mediated endocytosis (CME) are responsible for opposite intracellular trafficking, contributing to plasma membrane homeostasis. However, whether and how the two trafficking processes coordinate remain unclear. Here, we show that Kif5b directly interacts with clathrin heavy chain (CHC) at a region close to that for uncoating catalyst (Hsc70) and preferentially localizes on relatively large clathrin-coated vesicles (CCVs). Uncoating in vitro is decreased for CCVs from the cortex of kif5b conditional knockout (mutant) mouse and facilitated by adding Kif5b fragments containing CHC-binding site, while cell peripheral distribution of CHC or Hsc70 keeps unaffected by Kif5b depletion. Furthermore, cellular entry of vesicular stomatitis virus that internalizes into large CCV is inhibited by Kif5b depletion or introducing a dominant-negative Kif5b fragment. These findings showed a new role of Kif5b in regulating large CCV-mediated CME via affecting CCV uncoating, indicating Kif5b as a molecular knot connecting anterograde transport to CME. © 2018, The Author(s).

Original language	English
Article number	65
Journal	Cell Discovery
Volume	4
Issue number	1
DOIs	https://doi.org/10.1038/s41421-018-0067-5
Publication status	Published - 1 Dec 2018
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

This work was partially supported by Shenzhen Peacock project (KQTD2015033117210153); a National Basic Research Program of China (973 Program, 2014CB745200) from the Ministry of Science and Technology of PRC, and the Shenzhen Science and Technology Innovation Committee Basic Science Research Grant (JCYJ20150629151046896). The project was also supported by grants from the Hong Kong Research Grants Council (HKU 17127015, HKU 768113M) and the University of Hong Kong Small Project Fund to J.H. and Y.N.

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This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

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Ni, Y.-X., Zhou, N., Xue, W.-Q., Rong, L., Yung, W.-H., Lin, R.-Z., Kao, R. Y.-T., Duan, Z.-G., Sun, H.-T., Gong, H.-R., Tang, X.-M., Liu, M.-F., Zhang, W., Qi, S., Chung, S., Song, Y.-Q., & Huang, J.-D. (2018). A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating. Cell Discovery, 4(1), Article 65. https://doi.org/10.1038/s41421-018-0067-5

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title = "A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating",

abstract = "Kif5b-driven anterograde transport and clathrin-mediated endocytosis (CME) are responsible for opposite intracellular trafficking, contributing to plasma membrane homeostasis. However, whether and how the two trafficking processes coordinate remain unclear. Here, we show that Kif5b directly interacts with clathrin heavy chain (CHC) at a region close to that for uncoating catalyst (Hsc70) and preferentially localizes on relatively large clathrin-coated vesicles (CCVs). Uncoating in vitro is decreased for CCVs from the cortex of kif5b conditional knockout (mutant) mouse and facilitated by adding Kif5b fragments containing CHC-binding site, while cell peripheral distribution of CHC or Hsc70 keeps unaffected by Kif5b depletion. Furthermore, cellular entry of vesicular stomatitis virus that internalizes into large CCV is inhibited by Kif5b depletion or introducing a dominant-negative Kif5b fragment. These findings showed a new role of Kif5b in regulating large CCV-mediated CME via affecting CCV uncoating, indicating Kif5b as a molecular knot connecting anterograde transport to CME. {\textcopyright} 2018, The Author(s).",

author = "Yan-Xiang Ni and Nan Zhou and Wen-Qian Xue and Li Rong and Wing-Ho Yung and Rao-Zhou Lin and Kao, \{Richard Yi-Tsun\} and Zhi-Gang Duan and Hai-Tao Sun and Hua-Rui Gong and Xu-Ming Tang and Meng-Fei Liu and Wen Zhang and Shuang Qi and Sookja Chung and You-Qiang Song and Jian-Dong Huang",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].",

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Ni, Y-X, Zhou, N, Xue, W-Q, Rong, L, Yung, W-H, Lin, R-Z, Kao, RY-T, Duan, Z-G, Sun, H-T, Gong, H-R, Tang, X-M, Liu, M-F, Zhang, W, Qi, S, Chung, S, Song, Y-Q & Huang, J-D 2018, 'A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating', Cell Discovery, vol. 4, no. 1, 65. https://doi.org/10.1038/s41421-018-0067-5

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AU - Ni, Yan-Xiang

AU - Zhou, Nan

AU - Xue, Wen-Qian

AU - Rong, Li

AU - Yung, Wing-Ho

AU - Lin, Rao-Zhou

AU - Kao, Richard Yi-Tsun

AU - Duan, Zhi-Gang

AU - Sun, Hai-Tao

AU - Gong, Hua-Rui

AU - Tang, Xu-Ming

AU - Liu, Meng-Fei

AU - Zhang, Wen

AU - Qi, Shuang

AU - Chung, Sookja

AU - Song, You-Qiang

AU - Huang, Jian-Dong

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Kif5b-driven anterograde transport and clathrin-mediated endocytosis (CME) are responsible for opposite intracellular trafficking, contributing to plasma membrane homeostasis. However, whether and how the two trafficking processes coordinate remain unclear. Here, we show that Kif5b directly interacts with clathrin heavy chain (CHC) at a region close to that for uncoating catalyst (Hsc70) and preferentially localizes on relatively large clathrin-coated vesicles (CCVs). Uncoating in vitro is decreased for CCVs from the cortex of kif5b conditional knockout (mutant) mouse and facilitated by adding Kif5b fragments containing CHC-binding site, while cell peripheral distribution of CHC or Hsc70 keeps unaffected by Kif5b depletion. Furthermore, cellular entry of vesicular stomatitis virus that internalizes into large CCV is inhibited by Kif5b depletion or introducing a dominant-negative Kif5b fragment. These findings showed a new role of Kif5b in regulating large CCV-mediated CME via affecting CCV uncoating, indicating Kif5b as a molecular knot connecting anterograde transport to CME. © 2018, The Author(s).

AB - Kif5b-driven anterograde transport and clathrin-mediated endocytosis (CME) are responsible for opposite intracellular trafficking, contributing to plasma membrane homeostasis. However, whether and how the two trafficking processes coordinate remain unclear. Here, we show that Kif5b directly interacts with clathrin heavy chain (CHC) at a region close to that for uncoating catalyst (Hsc70) and preferentially localizes on relatively large clathrin-coated vesicles (CCVs). Uncoating in vitro is decreased for CCVs from the cortex of kif5b conditional knockout (mutant) mouse and facilitated by adding Kif5b fragments containing CHC-binding site, while cell peripheral distribution of CHC or Hsc70 keeps unaffected by Kif5b depletion. Furthermore, cellular entry of vesicular stomatitis virus that internalizes into large CCV is inhibited by Kif5b depletion or introducing a dominant-negative Kif5b fragment. These findings showed a new role of Kif5b in regulating large CCV-mediated CME via affecting CCV uncoating, indicating Kif5b as a molecular knot connecting anterograde transport to CME. © 2018, The Author(s).

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DO - 10.1038/s41421-018-0067-5

M3 - RGC 21 - Publication in refereed journal

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ER -

A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating

Abstract

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Funding

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