A new ratiometric two-photon fluorescent probe for imaging of lysosomes in living cells and tissues
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Pages (from-to) | 4637-4642 |
Journal / Publication | Tetrahedron |
Volume | 72 |
Issue number | 31 |
Online published | 18 Jun 2016 |
Publication status | Published - 4 Aug 2016 |
Link(s)
Abstract
Intracellular pH plays a pivotal role in various biological processes. Herein, we adopted the through bond energy transfer (TBET) strategy to design a unique type of ratiometric two-photon fluorescent probe for imaging of lysosomal pH in live cells and tissues, termed Np-Rh-Lys. Specifically, a two-photon fluorophore (D-π-A-structured naphthalimide derivative) and a rhodamine B fluorophore were directly connected, and dimethylamino moiety served as a lysosomal targeting-group. The experiments demonstrate new probe Np-Rh-Lys was a reliable and specific probe for labeling lysosomes in living cells with two well-resolved emission peaks separated by 80 nm, and which showed high ratiometric imaging resolution and deep-tissue imaging depth over 180 μm, we thus may expect the new platform prompt the development of a wide variety of ratiometric two-photon fluorescent probes application in biological systems.
Research Area(s)
- Fluorescent imaging, Lysosomal pH, Ratiometric fluorescent probe, Two-photon
Citation Format(s)
A new ratiometric two-photon fluorescent probe for imaging of lysosomes in living cells and tissues. / Zhou, Liyi; Liu, Yongchao; Hu, Shunqin et al.
In: Tetrahedron, Vol. 72, No. 31, 04.08.2016, p. 4637-4642.
In: Tetrahedron, Vol. 72, No. 31, 04.08.2016, p. 4637-4642.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review