TY - GEN
T1 - A new platform for manipulating a single dna molecule by using opticallay-induced dielectrophoresis
AU - Lin, Yen-Heng
AU - Chang, Chen-Min
AU - Lee, Gwo-Bin
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2009
Y1 - 2009
N2 - This paper presents a new platform capable of manipulating a single DNA molecule based on optically-induced dielectrophoretic (ODEP) forces. The ends of λ-phage DNA were bound with polystyrene beads which can be manipulated by optical images generated by a digital projector. The manipulation process of fluorescent-labeled DNA was observed in real time under the ODEP operation. Two kinds of DNA manipulation were conducted including DNA stretching and revolving through the computer-controlled optical images. The stretching force was also measured to estimate the manipulation ability of DNA molecule in this platform. The maximum stretching force was estimated to be about 49.73 pN when binding with 10 μm polystyrene beads. The developed platform may be a promising tool for single DNA molecule investigation. ©2009 IEEE.
AB - This paper presents a new platform capable of manipulating a single DNA molecule based on optically-induced dielectrophoretic (ODEP) forces. The ends of λ-phage DNA were bound with polystyrene beads which can be manipulated by optical images generated by a digital projector. The manipulation process of fluorescent-labeled DNA was observed in real time under the ODEP operation. Two kinds of DNA manipulation were conducted including DNA stretching and revolving through the computer-controlled optical images. The stretching force was also measured to estimate the manipulation ability of DNA molecule in this platform. The maximum stretching force was estimated to be about 49.73 pN when binding with 10 μm polystyrene beads. The developed platform may be a promising tool for single DNA molecule investigation. ©2009 IEEE.
KW - DNA manipulation
KW - DNA stretching
KW - Optically-induced dielectrophoresis
UR - https://www.scopus.com/pages/publications/71449096600
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-71449096600&origin=recordpage
U2 - 10.1109/SENSOR.2009.5285792
DO - 10.1109/SENSOR.2009.5285792
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 9781424441938
T3 - TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 1590
EP - 1593
BT - TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
T2 - TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Y2 - 21 June 2009 through 25 June 2009
ER -