A new and sensitive method for the quantification of HBV cccDNA by real-time PCR

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Jun Wu
  • Ying Chen
  • Marie C Lin
  • George K.K Lau
  • Hsiang-Fu Kung

Detail(s)

Original languageEnglish
Pages (from-to)1102-1107
Journal / PublicationBiochemical and Biophysical Research Communications
Volume295
Issue number5
Publication statusPublished - 2002
Externally publishedYes

Abstract

The persistence of covalently closed circular (ccc) DNA of Hepatitis B virus (HBV) in liver cells is believed to be the major reason for relapse after completion of HBV antiviral therapy. Up to now, there is no sensitive method to quantify cccDNA in infected liver cells. We designed a set of primers to specifically amplify DNA fragments from HBV cccDNA but not from viral genomic DNA. A good linear range was obtained when 100-107 copies of HBV cccDNA were used as template in the quantitative real-time PCR. Not only is this method rapid, economical, highly sensitive, it can be used to monitor HBV cccDNA in infected human liver biopsies and to guide patients undergoing long-term anti-HBV therapy. © 2002 Elsevier Science (USA). All rights reserved.

Research Area(s)

  • HBV cccDNA, HBV quantification, Hepatitis B virus, Quantitative PCR

Citation Format(s)

A new and sensitive method for the quantification of HBV cccDNA by real-time PCR. / He, Ming-Liang; Wu, Jun; Chen, Ying; Lin, Marie C; Lau, George K.K; Kung, Hsiang-Fu.

In: Biochemical and Biophysical Research Communications, Vol. 295, No. 5, 2002, p. 1102-1107.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review