A nanofluidic bioarray chip for fast and high-throughput detection of antibodies in biological fluids

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review

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Author(s)

Detail(s)

Original languageEnglish
Title of host publicationNanosystems in Engineering and Medicine
Volume8548
Publication statusPublished - 2012
Externally publishedYes

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8548
ISSN (Print)1605-7422

Conference

TitleNanosystems in Engineering and Medicine
PlaceKorea, Republic of
CityIncheon
Period10 - 12 September 2012

Abstract

Immunoassays have become a standard in secretome analysis in clinical and research analysis. In this field there is a need for a high throughput method that uses low sample volumes. Microfluidics and nanofluidics have been developed for this purpose. Our lab has developed a nanofluidic bioarray (NBA) chip with the goal being a high throughput system that assays low sample volumes against multiple probes. A combination of horizontal and vertical channels are produced to create an array antigens on the surface of the NBA chip in one dimension that is probed by flowing in the other dimension antibodies from biological fluids. We have tested the NBA chip by immobilizing streptavidin and then biotinylated peptide to detect the presence of a mouse monoclonal antibody (MAb) that is specific for the peptide. Bound antibody is detected by an AlexaFluor 647 labeled goat (anti-mouse IgG) polyclonal antibody. Using the NBA chip, we have successfully detected peptide binding by small-volume (0.5 μl) samples containing 50 attomoles (100 pM) MAb. © 2012 SPIE.

Research Area(s)

  • Biotinylated Peptide and Streptavidin, Enzyme-linked Immunosorbent Assay (ELISA), Fluorescent Immunoassay, Hemagglutinin, Mouse Ascites Fluid, Nanofluidics and Microfluidics, Polydimethylsiloxane (PDMS) Chips, Protein Bioarray

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to lbscholars@cityu.edu.hk.

Citation Format(s)

A nanofluidic bioarray chip for fast and high-throughput detection of antibodies in biological fluids. / Lee, Jonathan; Gulzar, Naveed; Scott, Jamie K.; Li, Paul C. H.

Nanosystems in Engineering and Medicine. Vol. 8548 2012. 85481Q (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8548).

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review