A mitochondrion-targeting turn-on fluorescent probe detection of endogenous hydroxyl radicals in living cells and zebrafish

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

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Author(s)

  • Gangqiang Yuan
  • Haiyuan Ding
  • Hongyan Sun
  • Liyi Zhou
  • Qinlu Lin

Detail(s)

Original languageEnglish
Article number126706
Journal / PublicationSensors and Actuators, B: Chemical
Volume296
Early online date18 Jun 2019
Publication statusPublished - 1 Oct 2019

Abstract

Detection and imaging of hydroxyl radical (OH) with high sensitivity and selectivity in biological systems is of great importance in evaluating the role of OH plays in the physiological and pathological processes. Herein, a novel mitochondrion-targeting turn-on fluorescent probe RThy, has been developed to detect OH in living cells and zebrafish. Probe's turn on mechanism is based on the cleavage of azo-bond by OH. In RThy sensing system, possessing two groups: a lipophilic cation as a targeting-group, and an azo group acts not only as a quenching-group for tunable light absorption but also as recognizing-group of OH. In the absence of OH, almost non-fluorescence emission at 550 nm was observed. However, after reaction with OH, bright fluorescence emission at 550 nm gradually increased with the continuous addition of OH, displaying ˜50 folds fluorescence intensity enhancements and a quite low detection limit of 8 nM. The selectivity test showed RThy exhibits excellent selectivity toward OH among all the ROS/RNS species tested. Furthermore, the probe has been successfully employed for imaging OH in living cells and zebrafish, thus demonstrating RThy as a useful tool for studying OH in practical sample application, and providing powerful theoretical basis and technical support for investigation of the interaction OH with physiological and pathology.

Research Area(s)

  • Bioimaging, Fluorescent probe, Hydroxyl radical (•OH), Mitochondrion-targeting, Zebrafish

Citation Format(s)