A Microfluidic System for Detection of Cholangiocarcinoma Cells by Using Heparan Sulfate Octasaccharides

Wei-Chun Tsai; Lien-Yu Hung; Teng-Yi Huang; Yen-Shen Shan; Shang-Cheng Hung; Gwo-Bin Lee

doi:10.1109/NEMS.2017.8017070

A Microfluidic System for Detection of Cholangiocarcinoma Cells by Using Heparan Sulfate Octasaccharides

Wei-Chun Tsai, Lien-Yu Hung, Teng-Yi Huang, Yen-Shen Shan, Shang-Cheng Hung^*, Gwo-Bin Lee^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

4 Citations (Scopus)

Abstract

This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.

Original language	English
Title of host publication	2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)
Publisher	IEEE
Pages	489-493
ISBN (Electronic)	978-1-5090-3059-0
DOIs	https://doi.org/10.1109/NEMS.2017.8017070
Publication status	Published - Apr 2017
Externally published	Yes
Event	12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 - UCLA, Los Angeles, United States Duration: 9 Apr 2017 → 12 Apr 2017 http://ieee-nems.org/2017/

Publication series

Name	IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)
Publisher	IEEE
ISSN (Electronic)	2474-3755

Conference

Conference	12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
Abbreviated title	IEEE-NEMS 2017
Country/Territory	United States
City	Los Angeles
Period	9/04/17 → 12/04/17
Internet address	http://ieee-nems.org/2017/

Research Keywords

biomarker
cell isolation
cholangiocarcinoma
heparan sulfate octasaccharide
microfluidics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/NEMS.2017.8017070

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Tsai, W.-C., Hung, L.-Y., Huang, T.-Y., Shan, Y.-S., Hung, S.-C., & Lee, G.-B. (2017). A Microfluidic System for Detection of Cholangiocarcinoma Cells by Using Heparan Sulfate Octasaccharides. In 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) (pp. 489-493). Article 8017070 (IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)). IEEE. https://doi.org/10.1109/NEMS.2017.8017070

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title = "A Microfluidic System for Detection of Cholangiocarcinoma Cells by Using Heparan Sulfate Octasaccharides",

abstract = "This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.",

keywords = "biomarker, cell isolation, cholangiocarcinoma, heparan sulfate octasaccharide, microfluidics",

author = "Wei-Chun Tsai and Lien-Yu Hung and Teng-Yi Huang and Yen-Shen Shan and Shang-Cheng Hung and Gwo-Bin Lee",

year = "2017",

month = apr,

doi = "10.1109/NEMS.2017.8017070",

language = "English",

series = "IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)",

publisher = "IEEE",

pages = "489--493",

booktitle = "2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)",

address = "United States",

note = "12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017, IEEE-NEMS 2017 ; Conference date: 09-04-2017 Through 12-04-2017",

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Tsai, W-C, Hung, L-Y, Huang, T-Y, Shan, Y-S, Hung, S-C & Lee, G-B 2017, A Microfluidic System for Detection of Cholangiocarcinoma Cells by Using Heparan Sulfate Octasaccharides. in 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)., 8017070, IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS), IEEE, pp. 489-493, 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017, Los Angeles, United States, 9/04/17. https://doi.org/10.1109/NEMS.2017.8017070

A Microfluidic System for Detection of Cholangiocarcinoma Cells by Using Heparan Sulfate Octasaccharides. / Tsai, Wei-Chun; Hung, Lien-Yu; Huang, Teng-Yi et al.
2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). IEEE, 2017. p. 489-493 8017070 (IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - A Microfluidic System for Detection of Cholangiocarcinoma Cells by Using Heparan Sulfate Octasaccharides

AU - Tsai, Wei-Chun

AU - Hung, Lien-Yu

AU - Huang, Teng-Yi

AU - Shan, Yen-Shen

AU - Hung, Shang-Cheng

AU - Lee, Gwo-Bin

PY - 2017/4

Y1 - 2017/4

N2 - This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.

AB - This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.

KW - biomarker

KW - cell isolation

KW - cholangiocarcinoma

KW - heparan sulfate octasaccharide

KW - microfluidics

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DO - 10.1109/NEMS.2017.8017070

M3 - RGC 32 - Refereed conference paper (with host publication)

T3 - IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)

SP - 489

EP - 493

BT - 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)

PB - IEEE

T2 - 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

Y2 - 9 April 2017 through 12 April 2017

ER -

Tsai WC, Hung LY, Huang TY, Shan YS, Hung SC, Lee GB. A Microfluidic System for Detection of Cholangiocarcinoma Cells by Using Heparan Sulfate Octasaccharides. In 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). IEEE. 2017. p. 489-493. 8017070. (IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)). doi: 10.1109/NEMS.2017.8017070

A Microfluidic System for Detection of Cholangiocarcinoma Cells by Using Heparan Sulfate Octasaccharides

Abstract

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Research Keywords

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