A microfluidic linear node array for the study of protein-ligand interactions

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

2 Scopus Citations
View graph of relations

Author(s)

  • Cheuk-Wing Li
  • Guodong Yu
  • Jingyun Jiang
  • Simon Ming-Yuen Lee
  • Changqing Yi
  • Wanqing Yue

Detail(s)

Original languageEnglish
Pages (from-to)3993-3999
Journal / PublicationLab on a Chip - Miniaturisation for Chemistry and Biology
Volume14
Issue number20
Online published25 Jul 2014
Publication statusPublished - 21 Oct 2014

Abstract

We have developed a microfluidic device for the continuous separation of small molecules from a protein mixture and demonstrated its practical use in the study of protein-ligand binding, a crucial aspect in drug discovery. Our results demonstrated dose-dependent binding between bovine serum albumin (BSA) and its small-molecule site marker, Eosin Y (EY), and found that the binding reached a plateau when the BSA : EY ratio was above 1, which agreed with the eosin binding capacity of BSA reported in literature. By streamline control using a combination of two fundamental building blocks (R and L nodes) with a microdevice operated at a high flow rate (up to 1300 μL h-1), a solution barrier was created to "filter" off protein/protein-ligand complexes such that the small unbound molecules were isolated and quantified easily. The percentage decrease of small molecules with increasing protein concentration indicated the presence of binding events. Several fluorophores with different molecular weights were used to test the performance of the microfluidic "filter", which was tunable by 1) the total flow rate, and/or 2) the flow distribution ratio between the two device inlets; both were easily controllable by changing the syringe pump settings. Since the microdevice was operated at a relatively high flow rate, aliquots were easily recovered from the device outlets to facilitate off-chip detection. This microfluidic design is a novel and promising tool for preliminary drug screening. © The Royal Society of Chemistry 2014

Citation Format(s)

A microfluidic linear node array for the study of protein-ligand interactions. / Li, Cheuk-Wing; Yu, Guodong; Jiang, Jingyun et al.
In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 14, No. 20, 21.10.2014, p. 3993-3999.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review