A microfluidic cell culture platform for real-time cellular imaging

Chia-Chun Hsieh; Song-Bin Huang; Ping-Ching Wu; Dar-Bin Shieh; Gwo-Bin Lee

doi:10.1007/s10544-009-9307-7

A microfluidic cell culture platform for real-time cellular imaging

Chia-Chun Hsieh
, Song-Bin Huang
, Ping-Ching Wu
, Dar-Bin Shieh
, Gwo-Bin Lee

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

33 Citations (Scopus)

Abstract

This study reports a new microfluidic cell culture platform for real-time, in vitro microscopic observation and evaluation of cellular functions. Microheaters, a micro temperature sensor, and micropumps are integrated into the system to achieve a self-contained, perfusion-based, cell culture microenvironment. The key feature of the platform includes a unique, ultra-thin, culture chamber with a depth of 180 μm, allowing for real-time, high-resolution cellular imaging by combining bright field and fluorescent optics to visualize nanoparticle-cell/ organelle interactions. The cell plating, culturing, harvesting and replenishing processes are performed automatically. The developed platform also enables drug screening and real-time, in situ investigation of the cellular and sub-cellular delivery process of nano vectors. The mitotic activity and the interaction between cells and the nano drug carriers (conjugated quantum dots-epirubicin) are successfully monitored in this device. This developed system could be a promising platform for a wide variety of applications such as high-throughput, cell-based studies and as a diagnostic cellular imaging system. © Springer Science+Business Media, LLC 2009.

Original language	English
Pages (from-to)	903-913
Journal	Biomedical Microdevices
Volume	11
Issue number	4
DOIs	https://doi.org/10.1007/s10544-009-9307-7
Publication status	Published - 2009
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Research Keywords

Cell culture
Cellular imaging
MEMS
Micro-bioreactors
Microfluidics
Nanoparticle

Access Output

10.1007/s10544-009-9307-7

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

@article{e92f6b1b42ae42309597202572bb65d9,

title = "A microfluidic cell culture platform for real-time cellular imaging",

abstract = "This study reports a new microfluidic cell culture platform for real-time, in vitro microscopic observation and evaluation of cellular functions. Microheaters, a micro temperature sensor, and micropumps are integrated into the system to achieve a self-contained, perfusion-based, cell culture microenvironment. The key feature of the platform includes a unique, ultra-thin, culture chamber with a depth of 180 μm, allowing for real-time, high-resolution cellular imaging by combining bright field and fluorescent optics to visualize nanoparticle-cell/ organelle interactions. The cell plating, culturing, harvesting and replenishing processes are performed automatically. The developed platform also enables drug screening and real-time, in situ investigation of the cellular and sub-cellular delivery process of nano vectors. The mitotic activity and the interaction between cells and the nano drug carriers (conjugated quantum dots-epirubicin) are successfully monitored in this device. This developed system could be a promising platform for a wide variety of applications such as high-throughput, cell-based studies and as a diagnostic cellular imaging system. {\textcopyright} Springer Science+Business Media, LLC 2009.",

keywords = "Cell culture, Cellular imaging, MEMS, Micro-bioreactors, Microfluidics, Nanoparticle",

author = "Chia-Chun Hsieh and Song-Bin Huang and Ping-Ching Wu and Dar-Bin Shieh and Gwo-Bin Lee",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].",

year = "2009",

doi = "10.1007/s10544-009-9307-7",

language = "English",

volume = "11",

pages = "903--913",

journal = "Biomedical Microdevices",

issn = "1387-2176",

publisher = "Springer",

number = "4",

}

TY - JOUR

T1 - A microfluidic cell culture platform for real-time cellular imaging

AU - Hsieh, Chia-Chun

AU - Huang, Song-Bin

AU - Wu, Ping-Ching

AU - Shieh, Dar-Bin

AU - Lee, Gwo-Bin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2009

Y1 - 2009

N2 - This study reports a new microfluidic cell culture platform for real-time, in vitro microscopic observation and evaluation of cellular functions. Microheaters, a micro temperature sensor, and micropumps are integrated into the system to achieve a self-contained, perfusion-based, cell culture microenvironment. The key feature of the platform includes a unique, ultra-thin, culture chamber with a depth of 180 μm, allowing for real-time, high-resolution cellular imaging by combining bright field and fluorescent optics to visualize nanoparticle-cell/ organelle interactions. The cell plating, culturing, harvesting and replenishing processes are performed automatically. The developed platform also enables drug screening and real-time, in situ investigation of the cellular and sub-cellular delivery process of nano vectors. The mitotic activity and the interaction between cells and the nano drug carriers (conjugated quantum dots-epirubicin) are successfully monitored in this device. This developed system could be a promising platform for a wide variety of applications such as high-throughput, cell-based studies and as a diagnostic cellular imaging system. © Springer Science+Business Media, LLC 2009.

AB - This study reports a new microfluidic cell culture platform for real-time, in vitro microscopic observation and evaluation of cellular functions. Microheaters, a micro temperature sensor, and micropumps are integrated into the system to achieve a self-contained, perfusion-based, cell culture microenvironment. The key feature of the platform includes a unique, ultra-thin, culture chamber with a depth of 180 μm, allowing for real-time, high-resolution cellular imaging by combining bright field and fluorescent optics to visualize nanoparticle-cell/ organelle interactions. The cell plating, culturing, harvesting and replenishing processes are performed automatically. The developed platform also enables drug screening and real-time, in situ investigation of the cellular and sub-cellular delivery process of nano vectors. The mitotic activity and the interaction between cells and the nano drug carriers (conjugated quantum dots-epirubicin) are successfully monitored in this device. This developed system could be a promising platform for a wide variety of applications such as high-throughput, cell-based studies and as a diagnostic cellular imaging system. © Springer Science+Business Media, LLC 2009.

KW - Cell culture

KW - Cellular imaging

KW - MEMS

KW - Micro-bioreactors

KW - Microfluidics

KW - Nanoparticle

UR - https://www.scopus.com/pages/publications/68249105490

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-68249105490&origin=recordpage

U2 - 10.1007/s10544-009-9307-7

DO - 10.1007/s10544-009-9307-7

M3 - RGC 21 - Publication in refereed journal

C2 - 19370417

SN - 1387-2176

VL - 11

SP - 903

EP - 913

JO - Biomedical Microdevices

JF - Biomedical Microdevices

IS - 4

ER -

A microfluidic cell culture platform for real-time cellular imaging

Abstract

Bibliographical note

Research Keywords

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Cite this