A microchip-based enzyme assay for lactate dehydrogenase isoenzymes

Gui-Sheng Zhuang; Jing Liu; Kang-Dong Liu; Chun-Ping Jia; Hui-Min Wang; Qing-Hui Jin; Jian-Long Zhao; Meng-Su Yang

A microchip-based enzyme assay for lactate dehydrogenase isoenzymes

Gui-Sheng Zhuang
, Jing Liu
, Kang-Dong Liu
, Chun-Ping Jia
, Hui-Min Wang
, Qing-Hui Jin
, Jian-Long Zhao
, Meng-Su Yang

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

1 Citation (Scopus)

Abstract

In this paper, a novel microchip-based electrophoresis analysis protocol of the lactate dehydrogenase (LDH) isoenzymes was developed. A Xe lamp-induced fluorescence detection system was set up to study on-chip LDH isoenzymes assay. Experiments for determination of LDH1 standard solution and serum LDH isoenzymes from a healthy adult donator were carried out, followed by on-chip monitoring of incubation product of NADH catalyzed by LDH. The efficient separation of different LDH forms indicates the potential of microfluidic devices for isoenzymes assay. This is the first work to assay LDH Isoenzymes with microchip-based capillary electrophoresis. Copyright © 2005 by the Transducer Research Foundation, Inc.

Original language	English
Title of host publication	Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Transducer Research Foundation
Pages	1318-1320
Volume	1
ISBN (Print)	0974361119, 9780974361116
Publication status	Published - 2005
Event	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States Duration: 9 Oct 2005 → 13 Oct 2005

Publication series

Name
Volume	1

Conference

Conference	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Place	United States
City	Boston, MA
Period	9/10/05 → 13/10/05

Research Keywords

Isoenzymes
Lactate dehydrogenase
Microchip capillary electrophoresis

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Zhuang, G.-S., Liu, J., Liu, K.-D., Jia, C.-P., Wang, H.-M., Jin, Q.-H., Zhao, J.-L., & Yang, M.-S. (2005). A microchip-based enzyme assay for lactate dehydrogenase isoenzymes. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (Vol. 1, pp. 1318-1320). Transducer Research Foundation.

@inproceedings{ed64de92a83749daa7376af94682534e,

title = "A microchip-based enzyme assay for lactate dehydrogenase isoenzymes",

abstract = "In this paper, a novel microchip-based electrophoresis analysis protocol of the lactate dehydrogenase (LDH) isoenzymes was developed. A Xe lamp-induced fluorescence detection system was set up to study on-chip LDH isoenzymes assay. Experiments for determination of LDH1 standard solution and serum LDH isoenzymes from a healthy adult donator were carried out, followed by on-chip monitoring of incubation product of NADH catalyzed by LDH. The efficient separation of different LDH forms indicates the potential of microfluidic devices for isoenzymes assay. This is the first work to assay LDH Isoenzymes with microchip-based capillary electrophoresis. Copyright {\textcopyright} 2005 by the Transducer Research Foundation, Inc.",

keywords = "Isoenzymes, Lactate dehydrogenase, Microchip capillary electrophoresis",

author = "Gui-Sheng Zhuang and Jing Liu and Kang-Dong Liu and Chun-Ping Jia and Hui-Min Wang and Qing-Hui Jin and Jian-Long Zhao and Meng-Su Yang",

year = "2005",

language = "English",

isbn = "0974361119",

volume = "1",

publisher = "Transducer Research Foundation",

pages = "1318--1320",

booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

note = "9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 ; Conference date: 09-10-2005 Through 13-10-2005",

}

Zhuang, G-S, Liu, J, Liu, K-D, Jia, C-P, Wang, H-M, Jin, Q-H, Zhao, J-L & Yang, M-S 2005, A microchip-based enzyme assay for lactate dehydrogenase isoenzymes. in Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. vol. 1, Transducer Research Foundation, pp. 1318-1320, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, United States, 9/10/05.

A microchip-based enzyme assay for lactate dehydrogenase isoenzymes. / Zhuang, Gui-Sheng; Liu, Jing; Liu, Kang-Dong et al.
Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1 Transducer Research Foundation, 2005. p. 1318-1320.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - A microchip-based enzyme assay for lactate dehydrogenase isoenzymes

AU - Zhuang, Gui-Sheng

AU - Liu, Jing

AU - Liu, Kang-Dong

AU - Jia, Chun-Ping

AU - Wang, Hui-Min

AU - Jin, Qing-Hui

AU - Zhao, Jian-Long

AU - Yang, Meng-Su

PY - 2005

Y1 - 2005

N2 - In this paper, a novel microchip-based electrophoresis analysis protocol of the lactate dehydrogenase (LDH) isoenzymes was developed. A Xe lamp-induced fluorescence detection system was set up to study on-chip LDH isoenzymes assay. Experiments for determination of LDH1 standard solution and serum LDH isoenzymes from a healthy adult donator were carried out, followed by on-chip monitoring of incubation product of NADH catalyzed by LDH. The efficient separation of different LDH forms indicates the potential of microfluidic devices for isoenzymes assay. This is the first work to assay LDH Isoenzymes with microchip-based capillary electrophoresis. Copyright © 2005 by the Transducer Research Foundation, Inc.

AB - In this paper, a novel microchip-based electrophoresis analysis protocol of the lactate dehydrogenase (LDH) isoenzymes was developed. A Xe lamp-induced fluorescence detection system was set up to study on-chip LDH isoenzymes assay. Experiments for determination of LDH1 standard solution and serum LDH isoenzymes from a healthy adult donator were carried out, followed by on-chip monitoring of incubation product of NADH catalyzed by LDH. The efficient separation of different LDH forms indicates the potential of microfluidic devices for isoenzymes assay. This is the first work to assay LDH Isoenzymes with microchip-based capillary electrophoresis. Copyright © 2005 by the Transducer Research Foundation, Inc.

KW - Isoenzymes

KW - Lactate dehydrogenase

KW - Microchip capillary electrophoresis

UR - https://www.scopus.com/pages/publications/84902478495

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84902478495&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 0974361119

SN - 9780974361116

VL - 1

SP - 1318

EP - 1320

BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Transducer Research Foundation

T2 - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005

Y2 - 9 October 2005 through 13 October 2005

ER -

A microchip-based enzyme assay for lactate dehydrogenase isoenzymes

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