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A magnetic-bead-based immunoassay for rapid purification and detection of influenza viruses utilizing suction-type microfluidic systems

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

Abstract

The study presents a magnetic-bead-based, suction-type microfluidic system for rapid purification and detection of influenza viral particles from clinical serum samples. Target influenza viral particles in the clinical sample can be first captured by the antibody-conjugated magnetic beads, followed by isolating the viral particles from the bio-samples with the incorporation of a permanent magnet and a suction-type microfluidic control module. Then, another specific secondary developing antibodies labeled with fluorescent dyes were employed to recognize the surface antigen of the virus-bound magnetic complexes. Finally, the fluorescent-labeled magnetic complexes can be then detected and analyzed by utilizing an optical detection module. The entire process can be performed automatically within in 30 minutes. The development of the integrated microfluidic chip may provide a promising platform for fast diagnosis of influenza infection.
Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages94-96
Volume1
Publication statusPublished - 2010
Externally publishedYes
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 3 Oct 20107 Oct 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume1

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
PlaceNetherlands
CityGroningen
Period3/10/107/10/10

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

Research Keywords

  • Immunoassay
  • Influenza
  • Magnetic bead
  • MEMS
  • Microfluidics
  • Suction

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