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A low temperature bonding of quartz microfluidic chip for serum lipoproteins analysis

  • Guisheng Zhuang
  • , Qinghui Jin
  • , Jing Liu
  • , Hui Cong
  • , Kangdong Liu
  • , Jianlong Zhao
  • , Mengsu Yang
  • , Huimin Wang

    Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

    Abstract

    A low-temperature bonding method for microfabrication of quartz microfluidic chips has been developed. The bonding process involved two steps: pre-bonding and post-annealing at low temperature. The bonding quality was evaluated by measuring the shear force at bonding interface and the electrical properties of the chips. Shear force of 5.66 MPa (566 N/cm2) was obtained in a bonded chip after a post-annealing at 200°C for 6 h. We owe the strong bonding strength to the formation of Si - O - Si bonds at the bonding interface during the post-annealing stage. The bonding procedures were not sensitive to surrounding and could be performed in a routine laboratory without clean room conditions. The performance of the fabricated microfluidic chips was tested by capillary zone electrophoresis (CZE) of serum lipoproteins with laser-induced fluorescence (LIF). The low-density (LDL) and high-density (HDL) lipoproteins in the serum was separated completely by using tricine buffer with methylglucamine. © Springer Science + Business Media, LLC 2006.
    Original languageEnglish
    Pages (from-to)255-261
    JournalBiomedical Microdevices
    Volume8
    Issue number3
    DOIs
    Publication statusPublished - Sept 2006

    UN SDGs

    This output contributes to the following UN Sustainable Development Goals (SDGs)

    1. SDG 9 - Industry, Innovation, and Infrastructure
      SDG 9 Industry, Innovation, and Infrastructure

    Research Keywords

    • Glass and/or quartz
    • Low temperature bonding
    • Microfluidic chip
    • Serum lipoprotein

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