A living cell array (LCA) for multiparameter cell metabolism studies

Sarah C. McQuaide; Mark R. Holl; Lloyd Burgess; Timothy Molter; Joseph Dragavon; A. Cody Young; Timothy Strovas; Judith Anderson; Alex Jen; Brandon Karlsgodt; Mary Lidstrom; Deirdre Meldrum

doi:10.1109/BIOROB.2006.1639217

A living cell array (LCA) for multiparameter cell metabolism studies

Sarah C. McQuaide, Mark R. Holl, Lloyd Burgess, Timothy Molter, Joseph Dragavon, A. Cody Young, Timothy Strovas, Judith Anderson, Alex Jen, Brandon Karlsgodt, Mary Lidstrom, Deirdre Meldrum

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

6 Citations (Scopus)

Abstract

A proof-of-platform-concept architecture for the analysis of living cell arrays is presented. Initial experiments conducted show a conceptual design for single-cell multiparameter measurements in real time. As a first parameter, extensive development and experimentation have been conducted on a sensor that will measure single-cell oxygen consumption. Wafer-level processing for chip fabrication and sensor deposition have been achieved, making possible the future goal of an automated system using disposable chips. The oxygen sensor has been tested for long-term adherence to a substrate in aqueous environments, biocompatibility with cell types of interest, and sensitivity to small changes in local oxygen concentration. A test device was made to verify the use of a gold foil barrier as an oxygen seal and shows good isolation of sealed areas.

Original language	English
Title of host publication	Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006
Pages	971-976
Volume	2006
DOIs	https://doi.org/10.1109/BIOROB.2006.1639217
Publication status	Published - 2006
Externally published	Yes
Event	1st IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006 - Pisa, Italy Duration: 20 Feb 2006 → 22 Feb 2006

Publication series

Name
Volume	2006

Conference

Conference	1st IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006
Place	Italy
City	Pisa
Period	20/02/06 → 22/02/06

Research Keywords

Microfabrication
Oxygen barrier
Oxygen consumption
Platinum porphyrin
Single-cell

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/BIOROB.2006.1639217

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McQuaide, S. C., Holl, M. R., Burgess, L., Molter, T., Dragavon, J., Young, A. C., Strovas, T., Anderson, J., Jen, A., Karlsgodt, B., Lidstrom, M., & Meldrum, D. (2006). A living cell array (LCA) for multiparameter cell metabolism studies. In Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006 (Vol. 2006, pp. 971-976). Article 1639217 https://doi.org/10.1109/BIOROB.2006.1639217

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title = "A living cell array (LCA) for multiparameter cell metabolism studies",

abstract = "A proof-of-platform-concept architecture for the analysis of living cell arrays is presented. Initial experiments conducted show a conceptual design for single-cell multiparameter measurements in real time. As a first parameter, extensive development and experimentation have been conducted on a sensor that will measure single-cell oxygen consumption. Wafer-level processing for chip fabrication and sensor deposition have been achieved, making possible the future goal of an automated system using disposable chips. The oxygen sensor has been tested for long-term adherence to a substrate in aqueous environments, biocompatibility with cell types of interest, and sensitivity to small changes in local oxygen concentration. A test device was made to verify the use of a gold foil barrier as an oxygen seal and shows good isolation of sealed areas.",

keywords = "Microfabrication, Oxygen barrier, Oxygen consumption, Platinum porphyrin, Single-cell",

author = "McQuaide, {Sarah C.} and Holl, {Mark R.} and Lloyd Burgess and Timothy Molter and Joseph Dragavon and Young, {A. Cody} and Timothy Strovas and Judith Anderson and Alex Jen and Brandon Karlsgodt and Mary Lidstrom and Deirdre Meldrum",

year = "2006",

doi = "10.1109/BIOROB.2006.1639217",

language = "English",

isbn = "1424400406",

volume = "2006",

pages = "971--976",

booktitle = "Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006",

note = "1st IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006 ; Conference date: 20-02-2006 Through 22-02-2006",

}

McQuaide, SC, Holl, MR, Burgess, L, Molter, T, Dragavon, J, Young, AC, Strovas, T, Anderson, J, Jen, A, Karlsgodt, B, Lidstrom, M & Meldrum, D 2006, A living cell array (LCA) for multiparameter cell metabolism studies. in Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006. vol. 2006, 1639217, pp. 971-976, 1st IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006, Pisa, Italy, 20/02/06. https://doi.org/10.1109/BIOROB.2006.1639217

A living cell array (LCA) for multiparameter cell metabolism studies. / McQuaide, Sarah C.; Holl, Mark R.; Burgess, Lloyd et al.
Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006. Vol. 2006 2006. p. 971-976 1639217.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - A living cell array (LCA) for multiparameter cell metabolism studies

AU - McQuaide, Sarah C.

AU - Holl, Mark R.

AU - Burgess, Lloyd

AU - Molter, Timothy

AU - Dragavon, Joseph

AU - Young, A. Cody

AU - Strovas, Timothy

AU - Anderson, Judith

AU - Jen, Alex

AU - Karlsgodt, Brandon

AU - Lidstrom, Mary

AU - Meldrum, Deirdre

PY - 2006

Y1 - 2006

N2 - A proof-of-platform-concept architecture for the analysis of living cell arrays is presented. Initial experiments conducted show a conceptual design for single-cell multiparameter measurements in real time. As a first parameter, extensive development and experimentation have been conducted on a sensor that will measure single-cell oxygen consumption. Wafer-level processing for chip fabrication and sensor deposition have been achieved, making possible the future goal of an automated system using disposable chips. The oxygen sensor has been tested for long-term adherence to a substrate in aqueous environments, biocompatibility with cell types of interest, and sensitivity to small changes in local oxygen concentration. A test device was made to verify the use of a gold foil barrier as an oxygen seal and shows good isolation of sealed areas.

AB - A proof-of-platform-concept architecture for the analysis of living cell arrays is presented. Initial experiments conducted show a conceptual design for single-cell multiparameter measurements in real time. As a first parameter, extensive development and experimentation have been conducted on a sensor that will measure single-cell oxygen consumption. Wafer-level processing for chip fabrication and sensor deposition have been achieved, making possible the future goal of an automated system using disposable chips. The oxygen sensor has been tested for long-term adherence to a substrate in aqueous environments, biocompatibility with cell types of interest, and sensitivity to small changes in local oxygen concentration. A test device was made to verify the use of a gold foil barrier as an oxygen seal and shows good isolation of sealed areas.

KW - Microfabrication

KW - Oxygen barrier

KW - Oxygen consumption

KW - Platinum porphyrin

KW - Single-cell

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U2 - 10.1109/BIOROB.2006.1639217

DO - 10.1109/BIOROB.2006.1639217

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 1424400406

SN - 9781424400409

VL - 2006

SP - 971

EP - 976

BT - Proceedings of the First IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006

T2 - 1st IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, 2006, BioRob 2006

Y2 - 20 February 2006 through 22 February 2006

ER -

A living cell array (LCA) for multiparameter cell metabolism studies

Abstract

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Research Keywords

UN SDGs

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