A lateral signalling pathway coordinates shape volatility during cell migration

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

16 Scopus Citations
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Author(s)

  • Valbona Luga
  • Sarah K. Armitage
  • Martin Musiol
  • Amy Won
  • Christopher M. Yip
  • Sergey V. Plotnikov
  • Jeffrey L. Wrana

Detail(s)

Original languageEnglish
Article number11714
Journal / PublicationNature Communications
Volume7
Publication statusPublished - 26 May 2016
Externally publishedYes

Link(s)

Abstract

Cell migration is fundamental for both physiological and pathological processes. Migrating cells usually display high dynamics in morphology, which is orchestrated by an integrative array of signalling pathways. Here we identify a novel pathway, we term lateral signalling, comprised of the planar cell polarity (PCP) protein Pk1 and the RhoGAPs, Arhgap21/23. We show that the Pk1-Arhgap21/23 complex inhibits RhoA, is localized on the non-protrusive lateral membrane cortex and its disruption leads to the disorganization of the actomyosin network and altered focal adhesion dynamics. Pk1-mediated lateral signalling confines protrusive activity and is regulated by Smurf2, an E3 ubiquitin ligase in the PCP pathway. Furthermore, we demonstrate that dynamic interplay between lateral and protrusive signalling generates cyclical fluctuations in cell shape that we quantify here as shape volatility, which strongly correlates with migration speed. These studies uncover a previously unrecognized lateral signalling pathway that coordinates shape volatility during productive cell migration.

Research Area(s)

Citation Format(s)

A lateral signalling pathway coordinates shape volatility during cell migration. / Zhang, Liang; Luga, Valbona; Armitage, Sarah K.; Musiol, Martin; Won, Amy; Yip, Christopher M.; Plotnikov, Sergey V.; Wrana, Jeffrey L.

In: Nature Communications, Vol. 7, 11714, 26.05.2016.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review