A high-resolution mitochondria-targeting ratiometric fluorescent probe for detection of the endogenous hypochlorous acid

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

21 Scopus Citations
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Author(s)

  • Liyi Zhou
  • Dan-Qing Lu
  • Qianqian Wang
  • Shunqin Hu
  • Haifei Wang
  • Xiaobing Zhang

Detail(s)

Original languageEnglish
Pages (from-to)129-134
Journal / PublicationSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume166
Online published16 May 2016
Publication statusPublished - 5 Sep 2016

Abstract

Hypochlorite anion, one of the biologically important reactive oxygen species, plays an essential role in diverse normal biochemical functions and abnormal pathological processes. Herein, an efficient high-resolution mitochondria-targeting ratiometric fluorescent probe for hypochlorous acid detection has been designed, synthesized and characterized. It is easily synthesized by the condensation reaction (C=C) of a 2-(2-hydroxyphenyl) quinazolin-4(3H)-one fluorophore and a cyanine group (mitochondria-targeting), which made the whole molecular a large Stokes shift (210 nm) and the two well-resolved emission peaks separated by 140 nm. As a result, it is considered as a good candidate for high resolution hypochlorous acid imaging in live cells. The ratiometric fluorescent probe exhibited outstanding features of high sensitivity, high selectivity, rapid response time (within 50 s), and excellent mitochondria-targeting ability. Moreover, the probe can also be successfully applied to imaging endogenously hypochlorous acid in the mitochondria of living cells with low cytotoxicity, and high resolution.

Research Area(s)

  • Fluorescent probe, HPQ-Cy2, Hypochlorous acid, Mitochondria, Ratiometric imaging

Citation Format(s)

A high-resolution mitochondria-targeting ratiometric fluorescent probe for detection of the endogenous hypochlorous acid. / Zhou, Liyi; Lu, Dan-Qing; Wang, Qianqian; Hu, Shunqin; Wang, Haifei; Sun, Hongyan; Zhang, Xiaobing.

In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, Vol. 166, 05.09.2016, p. 129-134.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal