A Graphene Oxide-based Covalent Resorufin-Conjugated Fluorescence “OFF-ON” Probe for Detection of Hydrazine

Qiang Guo; Jie Zhang; Hongyan Sun; Jian Lin Chen

doi:10.1002/asia.202200060

A Graphene Oxide-based Covalent Resorufin-Conjugated Fluorescence “OFF-ON” Probe for Detection of Hydrazine

Qiang Guo
, Jie Zhang
, Hongyan Sun^*
, Jian Lin Chen^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

2 Citations (Scopus)

Abstract

The unique properties of graphene oxide (GO), such as good water solubility, non-toxicity, biocompatibility and cell permeability, make GO well suited for a number of biological applications. In this study, we explore the application of GO as an efficient fluorescent quencher for a highly fluorescent organic dye, resorufin (RF). The quenching effect of GO on resorufin is studied by noncovalent and covalent bonding of resorufin, respectively. The fluorescence is completely quenched when resorufin is covalently linked to GO; while resorufin's fluorescence could be still observed through noncovalent bonding to GO sheet. Interestingly, addition of hydrazine into RF-GO complex causes the fluorescence recovered, providing a potential ‘‘OFF-ON’’ fluorescence responsive probe for detecting hydrazine in vitro and in living cells.

Original language	English
Article number	e202200060
Journal	Chemistry - An Asian Journal
Volume	17
Issue number	12
Online published	12 Apr 2022
DOIs	https://doi.org/10.1002/asia.202200060
Publication status	Published - 15 Jun 2022

Funding

This work was supported by Shenzhen Fundamental Research Program (JCYJ20190808181607387) and in part by Grants from Environment and Conservation Fund (ECF) of Hong Kong SAR, China (Project No. 2019-02) and the Research Grants Council of the Hong Kong SAR, China (UGC/IDS(R)16/19).

Research Keywords

covalent bonding
fluorescence quench
graphene oxide
hydrazine
resorufin

RGC Funding Information

RGC-funded

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10.1002/asia.202200060

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Cite this

@article{0aa97701646c4fccb3c5cf4342a4f346,

title = "A Graphene Oxide-based Covalent Resorufin-Conjugated Fluorescence “OFF-ON” Probe for Detection of Hydrazine",

abstract = "The unique properties of graphene oxide (GO), such as good water solubility, non-toxicity, biocompatibility and cell permeability, make GO well suited for a number of biological applications. In this study, we explore the application of GO as an efficient fluorescent quencher for a highly fluorescent organic dye, resorufin (RF). The quenching effect of GO on resorufin is studied by noncovalent and covalent bonding of resorufin, respectively. The fluorescence is completely quenched when resorufin is covalently linked to GO; while resorufin's fluorescence could be still observed through noncovalent bonding to GO sheet. Interestingly, addition of hydrazine into RF-GO complex causes the fluorescence recovered, providing a potential {\textquoteleft}{\textquoteleft}OFF-ON{\textquoteright}{\textquoteright} fluorescence responsive probe for detecting hydrazine in vitro and in living cells.",

keywords = "covalent bonding, fluorescence quench, graphene oxide, hydrazine, resorufin",

author = "Qiang Guo and Jie Zhang and Hongyan Sun and Chen, \{Jian Lin\}",

year = "2022",

month = jun,

day = "15",

doi = "10.1002/asia.202200060",

language = "English",

volume = "17",

journal = "Chemistry - An Asian Journal",

issn = "1861-4728",

publisher = "John Wiley \& Sons",

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TY - JOUR

T1 - A Graphene Oxide-based Covalent Resorufin-Conjugated Fluorescence “OFF-ON” Probe for Detection of Hydrazine

AU - Guo, Qiang

AU - Zhang, Jie

AU - Sun, Hongyan

AU - Chen, Jian Lin

PY - 2022/6/15

Y1 - 2022/6/15

N2 - The unique properties of graphene oxide (GO), such as good water solubility, non-toxicity, biocompatibility and cell permeability, make GO well suited for a number of biological applications. In this study, we explore the application of GO as an efficient fluorescent quencher for a highly fluorescent organic dye, resorufin (RF). The quenching effect of GO on resorufin is studied by noncovalent and covalent bonding of resorufin, respectively. The fluorescence is completely quenched when resorufin is covalently linked to GO; while resorufin's fluorescence could be still observed through noncovalent bonding to GO sheet. Interestingly, addition of hydrazine into RF-GO complex causes the fluorescence recovered, providing a potential ‘‘OFF-ON’’ fluorescence responsive probe for detecting hydrazine in vitro and in living cells.

AB - The unique properties of graphene oxide (GO), such as good water solubility, non-toxicity, biocompatibility and cell permeability, make GO well suited for a number of biological applications. In this study, we explore the application of GO as an efficient fluorescent quencher for a highly fluorescent organic dye, resorufin (RF). The quenching effect of GO on resorufin is studied by noncovalent and covalent bonding of resorufin, respectively. The fluorescence is completely quenched when resorufin is covalently linked to GO; while resorufin's fluorescence could be still observed through noncovalent bonding to GO sheet. Interestingly, addition of hydrazine into RF-GO complex causes the fluorescence recovered, providing a potential ‘‘OFF-ON’’ fluorescence responsive probe for detecting hydrazine in vitro and in living cells.

KW - covalent bonding

KW - fluorescence quench

KW - graphene oxide

KW - hydrazine

KW - resorufin

UR - https://www.scopus.com/pages/publications/85129240684

UR - https://www.scopus.com/pages/publications/85129240684#tab=impact

U2 - 10.1002/asia.202200060

DO - 10.1002/asia.202200060

M3 - RGC 21 - Publication in refereed journal

SN - 1861-4728

VL - 17

JO - Chemistry - An Asian Journal

JF - Chemistry - An Asian Journal

IS - 12

M1 - e202200060

ER -

A Graphene Oxide-based Covalent Resorufin-Conjugated Fluorescence “OFF-ON” Probe for Detection of Hydrazine

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ECF: A Biomicrofluidics for Monitoring Metabolic Activity in Anaerobic Co-digestion of Food Waste/Sewage Sludge

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A Graphene Oxide-based Covalent Resorufin-Conjugated Fluorescence “OFF-ON” Probe for Detection of Hydrazine

Abstract

Funding

Research Keywords

RGC Funding Information

Access Output

Other Access Options

Permanent Link

Other Files and Links

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ECF: A Biomicrofluidics for Monitoring Metabolic Activity in Anaerobic Co-digestion of Food Waste/Sewage Sludge

Cite this