A genomic strategy for cloning, expressing and purifying efflux proteins of the major facilitator superfamily

Gerda Szakonyi; Dong Leng; Pikyee Ma; Kim E. Bettaney; Massoud Saidijam; Alison Ward; Saeid Zibaei; Alastair T. Gardiner; Richard J. Cogdell; Patrick Butaye; Anne-Brit Kolsto; John O'Reilly; Ryan J. Hope; Nicholas G. Rutherford; Christopher J. Hoyle; Peter J.F. Henderson

doi:10.1093/jac/dkm036

Author(s)

Gerda Szakonyi
Dong Leng
Pikyee Ma
Kim E. Bettaney
Massoud Saidijam
Alison Ward
Saeid Zibaei
Alastair T. Gardiner
Richard J. Cogdell
Anne-Brit Kolsto
John O'Reilly
Ryan J. Hope
Nicholas G. Rutherford
Christopher J. Hoyle
Peter J.F. Henderson

Detail(s)

Original language	English
Pages (from-to)	1265-1270
Journal / Publication	Journal of Antimicrobial Chemotherapy
Volume	59
Issue number	6
Publication status	Published - Jun 2007
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1093/jac/dkm036 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-34447581023&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(f6a45410-7b22-4cc5-a7e8-5654e0219176).html

Abstract

A genomic strategy for the overexpression of bacterial multidrug and antibiotic resistance membrane efflux proteins in Escherichia coli is described. Expression is amplified so that the encoded proteins from a range of Gram-positive and Gram-negative bacteria comprise 5% to 35% of E. coli inner membrane protein. Depending upon their topology, proteins are produced with RGS(His)6-tag or a Strep-tag at the C terminus. These tags facilitate the purification of the overexpressed proteins in milligram quantities for structural studies. The strategy is illustrated for the bicyclomycin resistance efflux protein, Bcr, of E. coli. © The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.

Research Area(s)

Antibiotic resistance, Bicyclomycin, Brucella spp., Escherichia coli, Helicobacter pylori, Membrane transport proteins, Multidrugs, Pathogens

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Citation Format(s)

[ RIS ] [ BibTeX ]

A genomic strategy for cloning, expressing and purifying efflux proteins of the major facilitator superfamily. / Szakonyi, Gerda; Leng, Dong; Ma, Pikyee et al.
In: Journal of Antimicrobial Chemotherapy, Vol. 59, No. 6, 06.2007, p. 1265-1270.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Szakonyi, G, Leng, D, Ma, P, Bettaney, KE, Saidijam, M, Ward, A, Zibaei, S, Gardiner, AT, Cogdell, RJ, Butaye, P, Kolsto, A-B, O'Reilly, J, Hope, RJ, Rutherford, NG, Hoyle, CJ & Henderson, PJF 2007, 'A genomic strategy for cloning, expressing and purifying efflux proteins of the major facilitator superfamily', Journal of Antimicrobial Chemotherapy, vol. 59, no. 6, pp. 1265-1270. https://doi.org/10.1093/jac/dkm036

Szakonyi, G., Leng, D., Ma, P., Bettaney, K. E., Saidijam, M., Ward, A., Zibaei, S., Gardiner, A. T., Cogdell, R. J., Butaye, P., Kolsto, A.-B., O'Reilly, J., Hope, R. J., Rutherford, N. G., Hoyle, C. J., & Henderson, P. J. F. (2007). A genomic strategy for cloning, expressing and purifying efflux proteins of the major facilitator superfamily. Journal of Antimicrobial Chemotherapy, 59(6), 1265-1270. https://doi.org/10.1093/jac/dkm036

Szakonyi G, Leng D, Ma P, Bettaney KE, Saidijam M, Ward A et al. A genomic strategy for cloning, expressing and purifying efflux proteins of the major facilitator superfamily. Journal of Antimicrobial Chemotherapy. 2007 Jun;59(6):1265-1270. doi: 10.1093/jac/dkm036

Szakonyi, Gerda ; Leng, Dong ; Ma, Pikyee et al. / A genomic strategy for cloning, expressing and purifying efflux proteins of the major facilitator superfamily. In: Journal of Antimicrobial Chemotherapy. 2007 ; Vol. 59, No. 6. pp. 1265-1270.