A FRET-based ratiometric fluorescent probe for nitroxyl detection in living cells

Huatang Zhang; Ruochuan Liu; Yi Tan; William Haowei Xie; Haipeng Lei; Hon-Yeung Cheung; Hongyan Sun

doi:10.1021/am508987v

A FRET-based ratiometric fluorescent probe for nitroxyl detection in living cells

Huatang Zhang, Ruochuan Liu, Yi Tan, William Haowei Xie, Haipeng Lei, Hon-Yeung Cheung, Hongyan Sun^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

97 Citations (Scopus)

Abstract

HNO has recently been found to possess distinct biological functions from NO. Studying the biological functions of HNO calls for the development of sensitive and selective fluorescent probes. Herein, we designed and synthesized a FRET-based ratiometric probe to detect HNO in living cells. Our studies revealed that the probe is capable of detecting HNO in a rapid and ratiometric manner under physiological conditions. In bioimaging studies, the probe displayed a clear color change from blue to green when treated with HNO.

Original language	English
Pages (from-to)	5438-5443
Journal	ACS Applied Materials and Interfaces
Volume	7
Issue number	9
Online published	25 Feb 2015
DOIs	https://doi.org/10.1021/am508987v
Publication status	Published - 11 Mar 2015

Research Keywords

bioimaging
fluorescent probe
FRET probe
HNO
ratiometric

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title = "A FRET-based ratiometric fluorescent probe for nitroxyl detection in living cells",

abstract = "HNO has recently been found to possess distinct biological functions from NO. Studying the biological functions of HNO calls for the development of sensitive and selective fluorescent probes. Herein, we designed and synthesized a FRET-based ratiometric probe to detect HNO in living cells. Our studies revealed that the probe is capable of detecting HNO in a rapid and ratiometric manner under physiological conditions. In bioimaging studies, the probe displayed a clear color change from blue to green when treated with HNO.",

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T1 - A FRET-based ratiometric fluorescent probe for nitroxyl detection in living cells

AU - Zhang, Huatang

AU - Liu, Ruochuan

AU - Tan, Yi

AU - Xie, William Haowei

AU - Lei, Haipeng

AU - Cheung, Hon-Yeung

AU - Sun, Hongyan

PY - 2015/3/11

Y1 - 2015/3/11

N2 - HNO has recently been found to possess distinct biological functions from NO. Studying the biological functions of HNO calls for the development of sensitive and selective fluorescent probes. Herein, we designed and synthesized a FRET-based ratiometric probe to detect HNO in living cells. Our studies revealed that the probe is capable of detecting HNO in a rapid and ratiometric manner under physiological conditions. In bioimaging studies, the probe displayed a clear color change from blue to green when treated with HNO.

AB - HNO has recently been found to possess distinct biological functions from NO. Studying the biological functions of HNO calls for the development of sensitive and selective fluorescent probes. Herein, we designed and synthesized a FRET-based ratiometric probe to detect HNO in living cells. Our studies revealed that the probe is capable of detecting HNO in a rapid and ratiometric manner under physiological conditions. In bioimaging studies, the probe displayed a clear color change from blue to green when treated with HNO.

KW - bioimaging

KW - fluorescent probe

KW - FRET probe

KW - HNO

KW - ratiometric

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U2 - 10.1021/am508987v

DO - 10.1021/am508987v

M3 - RGC 21 - Publication in refereed journal

C2 - 25658137

SN - 1944-8244

VL - 7

SP - 5438

EP - 5443

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 9

ER -

A FRET-based ratiometric fluorescent probe for nitroxyl detection in living cells

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