A facile synthesis of silver nanoparticle with SERS and antimicrobial activity using Bacillus subtilis exopolysaccharides

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

31 Scopus Citations
View graph of relations

Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)1075-1087
Journal / PublicationJournal of Experimental Nanoscience
Volume9
Issue number10
Publication statusPublished - Nov 2014
Externally publishedYes

Abstract

In this study, we report a facile synthesis of silver nanoparticle having SERS and antimicrobial activity using bacterial exopolysaccharide (EPS). Bacillus subtilis (MTCC 2422) was grown in nutrient broth and the extracellular EPS secreted by the organism was extracted and purified. The purified EPS was used for the synthesis of silver nanoparticles. The kinetics of silver nanoparticle synthesis was deduced by varying the exposure time and the concentration of EPS. The rate constant (k) for the synthesis of silver nanoparticle was calculated from the slope of ln(A<sup>∞</sup> - A<sup>t</sup>) versus time plot. The k value was found to be 3.49 × 10<sup>-3</sup>, 5.81 × 10<sup>-3</sup> and 5.03 × 10<sup>-3</sup> per min for particle synthesis using 2, 5 and 10 mg/mL EPS, respectively. The nanoparticles synthesised had an average particle size of 5.18 ± 1.49 nm, 1.96 ± 0.77 nm and 2.08 ± 0.88 nm for 2, 5 and 10 mg/mL EPS, respectively. The synthesised particles were characterised using UV-Vis absorbance spectroscopy, high-resolution transmission electron microscopy (HRTEM) attached to EDS (energy dispersive spectroscopy), Fourier transform infrared spectroscopy (FTIR), surface enhanced Raman spectroscopy (SERS) and zeta potential analyser. To our knowledge, this is the first study to report SERS activity of microbial Bacillus subtilis EPS-based synthesis of silver nanoparticle. HRTEM images showed silver nanoparticle entrapped in polysaccharide nanocages. Silver nanoparticle showed higher adherence towards the bacterial surface, with good bactericidal activity against Pseudomonas aeroginosa and Staphylococcus aureus. © 2014 Copyright Taylor & Francis Group, LLC.

Research Area(s)

  • antimicrobial activity, exopolysaccharide, nanocages, SERS, silver nanoparticles

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to lbscholars@cityu.edu.hk.

Citation Format(s)

A facile synthesis of silver nanoparticle with SERS and antimicrobial activity using Bacillus subtilis exopolysaccharides. / Selvakumar, R.; Aravindh, S.; Ashok, Anuradha M. et al.
In: Journal of Experimental Nanoscience, Vol. 9, No. 10, 11.2014, p. 1075-1087.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review