A facile assay for direct colorimetric visualization of lipopolysaccharides at low nanomolar level

Jiayu Sun; Jiechao Ge; Weimin Liu; Xueliang Wang; Zhiyuan Fan; Wenwen Zhao; Hongyan Zhang; Pengfei Wang; Shuit-Tong Lee

doi:10.1007/s12274-012-0234-1

A facile assay for direct colorimetric visualization of lipopolysaccharides at low nanomolar level

Jiayu Sun
, Jiechao Ge
, Weimin Liu
, Xueliang Wang
, Zhiyuan Fan
, Wenwen Zhao
, Hongyan Zhang
, Pengfei Wang
, Shuit-Tong Lee

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

61 Citations (Scopus)

Abstract

We report a facile assay for the rapid visual detection of lipopolysaccharide (LPS) molecules down to the low nanomolar level by taking advantage of the electrostatic interaction between LPS molecules and cysteamine-modified gold nanoparticles (CSH-Au NPs). The large amount of negatively charged groups on the LPS molecules make LPS highly negatively charged. Thus, when modified with cysteamine, the positively charged gold nanoparticles can aggregate in the presence of trace amounts of LPS. The probe is simple, does not require any advanced instrumentation, and the limit of detection (LOD) was determined to be as low as 3. 3 × 10^-10 mol/L. To the best of our knowledge, it is the most sensitive synthetic LPS sensor reported so far. © 2012 Tsinghua University Press and Springer-Verlag Berlin Heidelberg.

Original language	English
Pages (from-to)	486-493
Journal	Nano Research
Volume	5
Issue number	7
DOIs	https://doi.org/10.1007/s12274-012-0234-1
Publication status	Published - Jul 2012
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

This work was supported by the National Natural Science Foundation of China (NSFC) (Grant Nos. 21073213, 60978034 and 20903110), the Main Direction Program of Knowledge Innovation of Chinese Academy of Sciences, and the National High Technology Research and Development Program of China (863 Program) (Grant No. 2009AA03Z318).

Research Keywords

endotoxin
gold nanoparticle
Gram-negative
Lipopolysaccharide (LPS)
sensor

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abstract = "We report a facile assay for the rapid visual detection of lipopolysaccharide (LPS) molecules down to the low nanomolar level by taking advantage of the electrostatic interaction between LPS molecules and cysteamine-modified gold nanoparticles (CSH-Au NPs). The large amount of negatively charged groups on the LPS molecules make LPS highly negatively charged. Thus, when modified with cysteamine, the positively charged gold nanoparticles can aggregate in the presence of trace amounts of LPS. The probe is simple, does not require any advanced instrumentation, and the limit of detection (LOD) was determined to be as low as 3. 3 × 10-10 mol/L. To the best of our knowledge, it is the most sensitive synthetic LPS sensor reported so far. {\textcopyright} 2012 Tsinghua University Press and Springer-Verlag Berlin Heidelberg.",

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AU - Fan, Zhiyuan

AU - Zhao, Wenwen

AU - Zhang, Hongyan

AU - Wang, Pengfei

AU - Lee, Shuit-Tong

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AB - We report a facile assay for the rapid visual detection of lipopolysaccharide (LPS) molecules down to the low nanomolar level by taking advantage of the electrostatic interaction between LPS molecules and cysteamine-modified gold nanoparticles (CSH-Au NPs). The large amount of negatively charged groups on the LPS molecules make LPS highly negatively charged. Thus, when modified with cysteamine, the positively charged gold nanoparticles can aggregate in the presence of trace amounts of LPS. The probe is simple, does not require any advanced instrumentation, and the limit of detection (LOD) was determined to be as low as 3. 3 × 10-10 mol/L. To the best of our knowledge, it is the most sensitive synthetic LPS sensor reported so far. © 2012 Tsinghua University Press and Springer-Verlag Berlin Heidelberg.

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KW - Gram-negative

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A facile assay for direct colorimetric visualization of lipopolysaccharides at low nanomolar level

Abstract

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Research Keywords

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