A dual-functioning adenoviral vector encoding both transforming growth factor-β3 and shRNA silencing type I collagen : Construction and controlled release for chondrogenesis

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

22 Scopus Citations
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Author(s)

  • Feng Zhang
  • Yongchang Yao
  • Jinghua Hao
  • Ruijie Zhou
  • Chengzheng Liu
  • Yihong Gong

Detail(s)

Original languageEnglish
Pages (from-to)70-77
Journal / PublicationJournal of Controlled Release
Volume142
Issue number1
Publication statusPublished - Feb 2010
Externally publishedYes

Abstract

Hyaline articular cartilage degeneration is a common clinical syndrome globally, whereas cell-based therapy has proved to be a good solution to such problems. Given that transforming growth factor (TGF-β3) is helpful in maintaining chondrocytic phenotype or stimulating chondrogenic differentiation of stem cells, vectors containing TGF-β3 expression cassette can be delivered to therapeutic cells. One problem involved in the application of therapeutic cells in chondrogenesis is the undesirable production of type I collagen in such cells as chondrocytes and synovial mesenchymal stem cells during ex vivo culture, which undermines the mechanical strength of engineered cartilage. RNA interference (RNAi) strategy can be used to knock down its expression to allow better biological and mechanical functions in artificial tissues. In this study, for the first time we report the construction of an adenoviral vector that can express both TGF-β3 to promote chondrogenesis and short hairpin RNA (shRNA) targeting type I collagen to block its production. This dual-functioning vector (Ad-dual) was found to function well in three model cell types: human fibroblast, osteoblast and porcine chondrocyte in terms of the release of TGF-β3 protein and down-regulation of type I collagen production. Besides, we also tested its efficacy in porcine synovial mesenchymal stem cells, highlighting its potential applications in cell-based therapy for the treatment of articular cartilage degeneration. © 2009 Elsevier B.V.

Research Area(s)

  • Adenovirus, Cartilage regeneration, Dual-functioning, RNA interference, Transforming growth factor

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to lbscholars@cityu.edu.hk.

Citation Format(s)

A dual-functioning adenoviral vector encoding both transforming growth factor-β3 and shRNA silencing type I collagen: Construction and controlled release for chondrogenesis. / Zhang, Feng; Yao, Yongchang; Hao, Jinghua et al.
In: Journal of Controlled Release, Vol. 142, No. 1, 02.2010, p. 70-77.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review