A compendium of DNA-binding specificities of transcription factors in Pseudomonas syringae

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

2 Scopus Citations
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Author(s)

  • Ligang Fan
  • Wenju Sun
  • Xiaoyu Li
  • Lucas Grunwald
  • Nan Wu
  • Xiaolong Shao
  • Yimeng Yin

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Detail(s)

Original languageEnglish
Article number4947
Journal / PublicationNature Communications
Volume11
Online published2 Oct 2020
Publication statusPublished - 2020

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Abstract

Pseudomonas syringae is a Gram-negative and model pathogenic bacterium that causes plant diseases worldwide. Here, we set out to identify binding motifs for all 301 annotated transcription factors (TFs) of P. syringae using HT-SELEX. We successfully identify binding motifs for 100 TFs. We map functional interactions between the TFs and their targets in virulence-associated pathways, and validate many of these interactions and functions using additional methods such as ChIP-seq, electrophoretic mobility shift assay (EMSA), RT-qPCR, and reporter assays. Our work identifies 25 virulence-associated master regulators, 14 of which had not been characterized as TFs before.

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Citation Format(s)

A compendium of DNA-binding specificities of transcription factors in Pseudomonas syringae. / Fan, Ligang; Wang, Tingting; Hua, Canfeng; Sun, Wenju; Li, Xiaoyu; Grunwald, Lucas; Liu, Jingui; Wu, Nan; Shao, Xiaolong; Yin, Yimeng; Yan, Jian; Deng, Xin.

In: Nature Communications, Vol. 11, 4947, 2020.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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