TY - JOUR
T1 - A comparative proteomic study on the effects of metal pollution in oysters Crassostrea hongkongensis
AU - Xu, Lanlan
AU - Ji, Chenglong
AU - Wu, Huifeng
AU - Tan, Qiaoguo
AU - Wang, Wen-Xiong
PY - 2016/11/15
Y1 - 2016/11/15
N2 - The metal pollution has posed great risk on the coastal organisms along the Jiulongjiang Estuary in South China. In this work, two-dimensional electrophoresis-based proteomics was applied to the oysters Crassostrea hongkongensis from metal pollution sites to characterize the proteomic responses to metal pollution. Metal accumulation and proteomic responses indicated that the oysters from BJ site were more severely contaminated than those from FG site. Compared with those oyster samples from the clean site (JZ), metal pollution induced cellular injuries, oxidative and immune stresses in oyster heapatopancreas from both BJ and FG sites via differential metabolic pathways. In addition, metal pollution in BJ site induced disturbance in energy and lipid metabolisms in oysters. Results indicated that cathepsin L and ferritin GF1 might be the biomarkers of As and Fe in oyster C. hongkongensis, respectively. This study demonstrates that proteomics is a useful tool for investigating biological effects induced by metal pollution.
AB - The metal pollution has posed great risk on the coastal organisms along the Jiulongjiang Estuary in South China. In this work, two-dimensional electrophoresis-based proteomics was applied to the oysters Crassostrea hongkongensis from metal pollution sites to characterize the proteomic responses to metal pollution. Metal accumulation and proteomic responses indicated that the oysters from BJ site were more severely contaminated than those from FG site. Compared with those oyster samples from the clean site (JZ), metal pollution induced cellular injuries, oxidative and immune stresses in oyster heapatopancreas from both BJ and FG sites via differential metabolic pathways. In addition, metal pollution in BJ site induced disturbance in energy and lipid metabolisms in oysters. Results indicated that cathepsin L and ferritin GF1 might be the biomarkers of As and Fe in oyster C. hongkongensis, respectively. This study demonstrates that proteomics is a useful tool for investigating biological effects induced by metal pollution.
KW - Crassostrea hongkongensis
KW - Hepatopancreas
KW - Metal pollution
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=84992593466&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84992593466&origin=recordpage
U2 - 10.1016/j.marpolbul.2016.07.009
DO - 10.1016/j.marpolbul.2016.07.009
M3 - RGC 21 - Publication in refereed journal
C2 - 27402499
SN - 0025-326X
VL - 112
SP - 436
EP - 442
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
IS - 1-2
ER -
