TY - JOUR
T1 - A CMOS/thin-film fluorescence contact imaging microsystem for DNA analysis
AU - Singh, Ritu Raj
AU - Ho, Derek
AU - Nilchi, Alireza
AU - Gulak, Glenn
AU - Yau, Patrick
AU - Genov, Roman
PY - 2010
Y1 - 2010
N2 - A hybrid CMOS/thin-film microsystem for fluorescence contact imaging is presented. The microsystem integrates a high-performance optical interference filter and a 128 × 128 pixel active pixel sensor fabricated in a standard 0.35-μm CMOS technology. The thin-film filter has an optical density greater than 6.0 at the wavelength of interest, providing adequate excitation rejection to the 532-nm solid-state laser. Microsystem performance is experimentally validated by imaging spots of Cyanine-3 fluorophore, conventionally used in DNA detection. The emission intensity as a function of fluorophore concentration is measured with an estimated sensitivity of 5000 fluorophore/μm2. A human DNA microarray has been imaged with the sensor prototype. © 2010 IEEE.
AB - A hybrid CMOS/thin-film microsystem for fluorescence contact imaging is presented. The microsystem integrates a high-performance optical interference filter and a 128 × 128 pixel active pixel sensor fabricated in a standard 0.35-μm CMOS technology. The thin-film filter has an optical density greater than 6.0 at the wavelength of interest, providing adequate excitation rejection to the 532-nm solid-state laser. Microsystem performance is experimentally validated by imaging spots of Cyanine-3 fluorophore, conventionally used in DNA detection. The emission intensity as a function of fluorophore concentration is measured with an estimated sensitivity of 5000 fluorophore/μm2. A human DNA microarray has been imaged with the sensor prototype. © 2010 IEEE.
KW - Contact imaging
KW - DNA detection
KW - Fluorescence imaging
KW - Microarray
KW - Optical filter
UR - http://www.scopus.com/inward/record.url?scp=77952957847&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-77952957847&origin=recordpage
U2 - 10.1109/TCSI.2010.2043990
DO - 10.1109/TCSI.2010.2043990
M3 - RGC 21 - Publication in refereed journal
SN - 1549-8328
VL - 57
SP - 1029
EP - 1038
JO - IEEE Transactions on Circuits and Systems I: Regular Papers
JF - IEEE Transactions on Circuits and Systems I: Regular Papers
IS - 5
M1 - 5439822
ER -