A chair-type G-quadruplex structure formed by a human telomeric variant DNA in K+ solution

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Changdong Liu
  • Bo Zhou
  • Yanyan Geng
  • Rui Feng
  • Haitao Miao
  • Naining Xu
  • Xiao Shi
  • Yingying You
  • Yuning Hong
  • Ben Zhong Tang
  • Vitaly Kuryavyi
  • Guang Zhu

Detail(s)

Original languageEnglish
Pages (from-to)218-226
Journal / PublicationChemical Science
Volume10
Issue number1
Online published4 Oct 2018
Publication statusPublished - 7 Jan 2019

Link(s)

Abstract

Guanine tracts of human telomeric DNA sequences are known to fold into eight different four-stranded structures that vary by the conformation of guanine nucleotides arranged in the stack of G-tetrads in their core and by different kinds and orders of connecting loops, called G-quadruplexes. Here, we present a novel G-quadruplex structure formed in K+ solution by a human telomeric variant d[(GGGTTA)2GGGTTTGGG], htel21T18. This variant DNA is located in the subtelomeric regions of human chromosomes 8, 11, 17, and 19 as well as in the DNase hypersensitive region and in the subcentromeric region of chromosome 5. Interestingly, single A18T substitution that makes htel21T18 different from the human telomeric sequence results in the formation of a three-layer chair-type G-quadruplex, a fold previously unknown among human telomeric repeats, with two loops interacting through the reverse Watson-Crick A6·T18 base pair. The loops are edgewise; glycosidic conformation of guanines is syn·anti·syn·anti around each tetrad, and each strand of the core has two antiparallel adjacent strands. Our results expand the repertoire of known G-quadruplex folding topologies and may provide a potential target for structure-based anticancer drug design.

Research Area(s)

Citation Format(s)

A chair-type G-quadruplex structure formed by a human telomeric variant DNA in K+ solution. / Liu, Changdong; Zhou, Bo; Geng, Yanyan; Yan Tam, Dick; Feng, Rui; Miao, Haitao; Xu, Naining; Shi, Xiao; You, Yingying; Hong, Yuning; Tang, Ben Zhong; Kwan Lo, Pik; Kuryavyi, Vitaly; Zhu, Guang.

In: Chemical Science, Vol. 10, No. 1, 07.01.2019, p. 218-226.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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