A Cell Permeable NPE Caged ADP-Ribose for Studying TRPM2

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Peilin Yu
  • Qian Wang
  • Li-He Zhang
  • Hon-Cheung Lee
  • Liangren Zhang

Detail(s)

Original languageEnglish
Article numbere51028
Journal / PublicationPLoS ONE
Volume7
Issue number12
Publication statusPublished - 7 Dec 2012
Externally publishedYes

Abstract

Transient potential receptor melastatin-2 (TRPM2) is a non-selective Ca2+-permeable cation channel of the TRPM channel subfamily and is mainly activated by intracellular adenosine diphosphate ribose (ADPR). Here we synthesized a 1-(2-nitrophenyl)ethyl caged ADPR (NPE-ADPR) and found that uncaging of NPE-ADPR efficiently stimulated Ca2+, Mg2+, and Zn2+ influx in a concentration-dependent manner in intact human Jurkat T-lymphocytes. The cation influx was inhibited by inhibitors or knockdown of TRPM2. Likewise, uncaging of NPE-ADPR markedly induced cation entry in HEK 293 cells that overexpress TRPM2. As expected, high temperature increased the ability of the photolyzed NPE-ADPR to induce cation entry, whereas acidic pH inhibited. Moreover, the absence of extracellular Ca2+ significantly inhibited Mg2+ and Zn2+ influx after uncaging NPE-ADPR. On the other hand, the absence of extracellular Na+ or Mg2+ had no effect on photolyzed NPE-ADPR induced Ca2+ entry. Taken together, our results indicated that NPE-ADPR is a cell permeable ADPR analogue that is useful for studying TRPM2-mediated cation entry in intact cells. © 2012 Yu et al.

Citation Format(s)

A Cell Permeable NPE Caged ADP-Ribose for Studying TRPM2. / Yu, Peilin; Wang, Qian; Zhang, Li-He; Lee, Hon-Cheung; Zhang, Liangren; Yue, Jianbo.

In: PLoS ONE, Vol. 7, No. 12, e51028, 07.12.2012.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal