TY - JOUR
T1 - A biocompatible electrochemical sensor based on PtNi alloy nanoparticles-coupled N-GQDs for in situ monitoring of dopamine in glioma cells
AU - Panda, A.K.
AU - Murugan, K.
AU - Sakthivel, R.
AU - Dhawan, U.
AU - Lin, L.-Y.
AU - Duann, Y.-F.
AU - He, J.-H.
AU - Chung, R.-J.
PY - 2023/1
Y1 - 2023/1
N2 - We report the design and construction of enzyme-free sensor using platinum–nickel (PtNi) bimetallic alloy nanoparticle-conjugated nitrogen-doped graphene quantum dots (N-GQDs) for the highly specific in situ monitoring of dopamine (DA) secreted by glioma cells (C6). PtNi@N-GQDs nanocomposites were synthesized using a simple ultrasonication method. The resulting hybrid material was an excellent electrocatalyst for the redox activity of DA owing to the combined properties of PtNi alloys and highly conductive N-GQDs. The PtNi@N-GQDs-based sensing platform demonstrated substantial sensing ability with a detection range of 0.0125–952 μM, a sensitivity of 0.279 μA/μM/cm2, and a limit of detection of 0.005 μM (S/N = 3). The sensing performance of PtNi@N-GQDs was highly stable, selective, and reproducible. We successfully showed the practical application of the PtNi@N-GQDs sensor by quantifying DA in the blood serum and human urine samples. Finally, we used the PtNi@N-GQDs biocompatible platform to quantify DA released from C6 cells.
AB - We report the design and construction of enzyme-free sensor using platinum–nickel (PtNi) bimetallic alloy nanoparticle-conjugated nitrogen-doped graphene quantum dots (N-GQDs) for the highly specific in situ monitoring of dopamine (DA) secreted by glioma cells (C6). PtNi@N-GQDs nanocomposites were synthesized using a simple ultrasonication method. The resulting hybrid material was an excellent electrocatalyst for the redox activity of DA owing to the combined properties of PtNi alloys and highly conductive N-GQDs. The PtNi@N-GQDs-based sensing platform demonstrated substantial sensing ability with a detection range of 0.0125–952 μM, a sensitivity of 0.279 μA/μM/cm2, and a limit of detection of 0.005 μM (S/N = 3). The sensing performance of PtNi@N-GQDs was highly stable, selective, and reproducible. We successfully showed the practical application of the PtNi@N-GQDs sensor by quantifying DA in the blood serum and human urine samples. Finally, we used the PtNi@N-GQDs biocompatible platform to quantify DA released from C6 cells.
KW - Dopamine
KW - Enzyme-free sensor
KW - Glioma cells
KW - N-doped graphene quantum dots
KW - Neurotransmitter
UR - http://www.scopus.com/inward/record.url?scp=85143542585&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85143542585&origin=recordpage
U2 - 10.1016/j.mtchem.2022.101283
DO - 10.1016/j.mtchem.2022.101283
M3 - RGC 21 - Publication in refereed journal
SN - 2468-5194
VL - 27
JO - Materials Today Chemistry
JF - Materials Today Chemistry
M1 - 101283
ER -