A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Chi-Kin Koo
  • Ka-Leung Wong
  • Cornelia Wing-Yin Man
  • Leo King-Yan So
  • Hoi-Lam Tam
  • Sai-Wah Tsao
  • Kok-Wai Cheah
  • Yang-Yi Yang
  • Jin-Can Chen
  • Michael Hon-Wah Lam

Detail(s)

Original languageEnglish
Pages (from-to)872-878
Journal / PublicationInorganic Chemistry
Volume48
Issue number3
Publication statusPublished - 2 Feb 2009

Abstract

The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h. © 2009 American Chemical Society.

Citation Format(s)

A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging. / Koo, Chi-Kin; Wong, Ka-Leung; Man, Cornelia Wing-Yin; Lam, Yun-Wah; So, Leo King-Yan; Tam, Hoi-Lam; Tsao, Sai-Wah; Cheah, Kok-Wai; Lau, Kai-Chung; Yang, Yang-Yi; Chen, Jin-Can; Lam, Michael Hon-Wah.

In: Inorganic Chemistry, Vol. 48, No. 3, 02.02.2009, p. 872-878.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review