6353D Microstructures to Realize Single Cell Culture on Digital Microfluidic Chip for Precise Medicine

Jiao Zhai; Yunyi Li; Cheng Dong; Haoran Li; Yanwei Jia; Pui-in Mak; Rui P. Martins

6353D Microstructures to Realize Single Cell Culture on Digital Microfluidic Chip for Precise Medicine

Jiao Zhai
, Yunyi Li
, Cheng Dong
, Haoran Li
, Yanwei Jia^*
, Pui-in Mak
, Rui P. Martins

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

This paper reports a simple microfabrication method for single cell culture on a digital microfluidics chip. The method is based on featuring 3D microstructure array on the dielectric layer of the chip. MDA-MB-231 cells were shown to trap in the 3D microstructure array with single cell efficiency to 20%. Based on the designed microstructures, we used Cisplatin (Cis) as model to measure toxicity to breast cancer cells MDA-MB-231 on our-chip and off-chip. The results suggest that the designed structures are effective for single cell trapping, and evaluation of drug toxicity over time.

Original language	English
Title of host publication	μTAS2018
Publisher	Chemical and Biological Microsystems Society
Pages	1315-1317
ISBN (Electronic)	9780578405308
ISBN (Print)	9781510897571
Publication status	Published - Nov 2018
Externally published	Yes
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018) - Kaohsiung Exhibition Center, Kaohsiung, Taiwan, China Duration: 11 Nov 2018 → 15 Nov 2018 https://cbmsociety.org/microtas/microtas2018/ https://cbmsociety.org/wp-content/uploads/2018/11/uTAS-2018-FInal-Program-WEB.pdf https://archive.cbmsociety.org/utas/uTAS2018.zip

Publication series

Name	International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS
Publisher	Chemical and Biological Microsystems Society
ISSN (Electronic)	1556-5904

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)
Abbreviated title	μTAS2018
Place	Taiwan, China
City	Kaohsiung
Period	11/11/18 → 15/11/18
Internet address	https://cbmsociety.org/microtas/microtas2018/ https://cbmsociety.org/wp-content/uploads/2018/11/uTAS-2018-FInal-Program-WEB.pdf https://archive.cbmsociety.org/utas/uTAS2018.zip

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being
SDG 9 Industry, Innovation, and Infrastructure

Research Keywords

Digital microfluidics
Drug screening
Single cell culture

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@inproceedings{225a8f698eae4a08a98b2b4203ea6d74,

title = "6353D Microstructures to Realize Single Cell Culture on Digital Microfluidic Chip for Precise Medicine",

abstract = "This paper reports a simple microfabrication method for single cell culture on a digital microfluidics chip. The method is based on featuring 3D microstructure array on the dielectric layer of the chip. MDA-MB-231 cells were shown to trap in the 3D microstructure array with single cell efficiency to 20\%. Based on the designed microstructures, we used Cisplatin (Cis) as model to measure toxicity to breast cancer cells MDA-MB-231 on our-chip and off-chip. The results suggest that the designed structures are effective for single cell trapping, and evaluation of drug toxicity over time.",

keywords = "Digital microfluidics, Drug screening, Single cell culture, Digital microfluidics, Drug screening, Single cell culture, Digital microfluidics, Drug screening, Single cell culture",

author = "Jiao Zhai and Yunyi Li and Cheng Dong and Haoran Li and Yanwei Jia and Pui-in Mak and Martins, \{Rui P.\}",

year = "2018",

month = nov,

language = "English",

isbn = "9781510897571",

series = "International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS",

publisher = "Chemical and Biological Microsystems Society",

pages = "1315--1317",

booktitle = "μTAS2018",

note = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018), μTAS2018 ; Conference date: 11-11-2018 Through 15-11-2018",

url = "https://cbmsociety.org/microtas/microtas2018/, https://cbmsociety.org/wp-content/uploads/2018/11/uTAS-2018-FInal-Program-WEB.pdf, https://archive.cbmsociety.org/utas/uTAS2018.zip",

}

Zhai, J, Li, Y, Dong, C, Li, H, Jia, Y, Mak, P & Martins, RP 2018, 6353D Microstructures to Realize Single Cell Culture on Digital Microfluidic Chip for Precise Medicine. in μTAS2018. International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS, Chemical and Biological Microsystems Society, pp. 1315-1317, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018), Kaohsiung, Taiwan, China, 11/11/18.

6353D Microstructures to Realize Single Cell Culture on Digital Microfluidic Chip for Precise Medicine. / Zhai, Jiao; Li, Yunyi; Dong, Cheng et al.
μTAS2018. Chemical and Biological Microsystems Society, 2018. p. 1315-1317 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - 6353D Microstructures to Realize Single Cell Culture on Digital Microfluidic Chip for Precise Medicine

AU - Zhai, Jiao

AU - Li, Yunyi

AU - Dong, Cheng

AU - Li, Haoran

AU - Jia, Yanwei

AU - Mak, Pui-in

AU - Martins, Rui P.

PY - 2018/11

Y1 - 2018/11

N2 - This paper reports a simple microfabrication method for single cell culture on a digital microfluidics chip. The method is based on featuring 3D microstructure array on the dielectric layer of the chip. MDA-MB-231 cells were shown to trap in the 3D microstructure array with single cell efficiency to 20%. Based on the designed microstructures, we used Cisplatin (Cis) as model to measure toxicity to breast cancer cells MDA-MB-231 on our-chip and off-chip. The results suggest that the designed structures are effective for single cell trapping, and evaluation of drug toxicity over time.

AB - This paper reports a simple microfabrication method for single cell culture on a digital microfluidics chip. The method is based on featuring 3D microstructure array on the dielectric layer of the chip. MDA-MB-231 cells were shown to trap in the 3D microstructure array with single cell efficiency to 20%. Based on the designed microstructures, we used Cisplatin (Cis) as model to measure toxicity to breast cancer cells MDA-MB-231 on our-chip and off-chip. The results suggest that the designed structures are effective for single cell trapping, and evaluation of drug toxicity over time.

KW - Digital microfluidics

KW - Drug screening

KW - Single cell culture

KW - Digital microfluidics

KW - Drug screening

KW - Single cell culture

KW - Digital microfluidics

KW - Drug screening

KW - Single cell culture

UR - https://www.scopus.com/pages/publications/85079738066

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85079738066&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781510897571

T3 - International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS

SP - 1315

EP - 1317

BT - μTAS2018

PB - Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)

Y2 - 11 November 2018 through 15 November 2018

ER -

6353D Microstructures to Realize Single Cell Culture on Digital Microfluidic Chip for Precise Medicine

Abstract

Publication series

Conference

UN SDGs

Research Keywords

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