6 种淫羊藿黄酮抗氧化和抗肿瘤活性的比较

Objective: To study the antioxidative and antitumor activities of flavonoids isolated from Epimedium koreanum. Method: The compounds were separated by column chromatography with silica gel and Sephadex LH-20, and identified by spectral a nalysis (ESI-MS, ¹H-NMR and ¹³C-NMR) respectively. DPPH radical scavenging assay and MTT assay were used to observe the antioxidative and antitumor abilities. Result: Six compounds were isolated from the the ethyl acetate extract of the aerial part. Their structures were identified as icariin (I), luteolin (II), baohuoside II (III), hyperoside (IV), epimedokoreanin B (V) and baohuoside I (VI). The results indicated that at concentrations of 3. 125-200 μmol · L^-1, compound I, III and VI had no ability to scavenge the DPPH radical, but the scavenging ability of compounds II , IV and V were stronger than that of Vit c in dose-dependant manner. Compounds I, II, V and VI could inhibit the proliferation of MCF-7 and HepG2 in dose-dependant manner, but compounds III and IV had no effect on the proliferation. Conclusion: The antitumor activity of E. koreanum may be partially related to the antioxidantive activity of flavonoids.