3-Oxoacyl acyl carrier protein reductase overexpression reveals its unprecedented roles in biofuel production and high-temperature tolerance in diatom

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

View graph of relations

Author(s)

  • Guang-Bin Ye
  • Xiao-Yun Bin
  • Hong-Ye Li
  • Xiang Wang

Related Research Unit(s)

Detail(s)

Original languageEnglish
Article number124844
Journal / PublicationFuel
Volume325
Online published18 Jun 2022
Publication statusPublished - 1 Oct 2022

Abstract

Due to its high lipid accumulation capability, Phaeodactylum tricornutum has attracted considerable attention as a promising candidate for biofuel production. However, the derived lipid cannot satisfy the industrial biofuel manufacturing requirements given its poor fatty acid characteristics. Genetic engineering, as a prospective approach, has been employed to overcome this bottleneck. Relevant research indicates that the fabG gene plays a crucial role in fatty acid biosynthesis in bacteria, but its significance in microalgae remains unclear. In this study, FabG was successfully introduced, transcribed, and overexpressed in P. tricornutum. FabG overexpression significantly enhanced lipid (1.49-fold) and fatty acid (1.31-fold) biosynthesis without altering growth and photosynthesis, and this method thus exhibits great potential in high-quality biodiesel production. Moreover, increased saturation of fatty acids (e.g., C16:0) and improved antioxidative activities enhanced the tolerance of transgenic P. tricornutum to high temperature stress. To conclude, this study reports a feasible approach for high-quality algae-based biofuel production via the unprecedented roles of FabG retrieved from diatom.

Research Area(s)

  • 3-Oxoacyl acyl carrier protein reductase, Biofuel, Diatom, Fatty acid, Stress tolerance

Citation Format(s)

3-Oxoacyl acyl carrier protein reductase overexpression reveals its unprecedented roles in biofuel production and high-temperature tolerance in diatom. / Ye, Guang-Bin; Qin, Zi-Hao; Bin, Xiao-Yun; Mou, Jin-Hua; Lin, Carol Sze Ki; Li, Hong-Ye; Wang, Xiang.

In: Fuel, Vol. 325, 124844, 01.10.2022.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review