3D Cell Assembly based on the Manipulation of Electrode towards via Electoral Deposition

Cell culture is a key technique for tissue engineering and the creation of organs. Many cell culture works have been studied up to now. However, these works are mainly focusing on the cell culture on a plate. Therefore, the cells can only growth on a flat plate, which environment is different from in vivo. Hydrogel is a kind of biocompatible material, inside with the cell can spread and growth. It provides us a method to study the cell’s property in real 3D environment. Thus, how to control the shape of the gel is an important topic. In this paper, we proposed a direct writing method to control the gel’s shape and position based on micro manipulation. The gel generation is based on the electrodeposition of calcium alginate. A “NU” shape was generated by the proposed method to show how our system is working.