Abstract
A novel incubation and activity determination method of lactate dehydrogenase isoenzymes was established. Based on the on-chip electrophoretic separation theory the analyzer was integrated with a temperature-controlling system. The assay program of the enzymes including injection of samples, incubation and separation was highly performed in the microchannel. A system for high sensitivity fluorescence detection was developed. A 360 nm light source was adopted to excite the fluorescence and the 460 nm signature was finally selectively gathered. The efficient separation of different LDH fractions indicates the potential of microfluidic devices for isoenzymes assay and the detection limit reached 0.5 U/L.
| Translated title of the contribution | Incubation and Activity Determination of Lactate Dehydrogenase Isoenzymes on Microfluidic Chip |
|---|---|
| Original language | Chinese (Simplified) |
| Pages (from-to) | 1223-1226 |
| Journal | 高等学校化学学报 |
| Volume | 27 |
| Issue number | 7 |
| Publication status | Published - Jul 2006 |
| Externally published | Yes |
Research Keywords
- 微流控芯片
- 温控
- 同工酶
- 活性检测
- Microfluidic chip
- Temperature-controlling
- Lactate dehydrogenase isoenzymes
- Activity determination
Fingerprint
Dive into the research topics of 'Incubation and Activity Determination of Lactate Dehydrogenase Isoenzymes on Microfluidic Chip'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver